Voltage Imaging of Cortical Oscillations in Layer 1 with Two-Photon Microscopy

抄録

Membrane voltage oscillations in layer 1 (L1) of primary sensory cortices might be important indicators of cortical gain control, attentional focusing, and signal integration. However, electric field recordings are hampered by the low seal resistance of electrodes close to the brain surface. To study L1 membrane voltage oscillations, we synthesized a new voltage-sensitive dye, di1-ANNINE-6plus, that can diffuse into tissue. We applied it with a new surgery, leaving the dura intact but allowing injection of large quantities of staining solution, and imaged cortical membrane potential oscillations with two-photon microscopy depth-resolved (25 to 100 microm below dura) in anesthetized and awake mice. We found delta (0.5-4 Hz), theta (4-10 Hz), low beta (10-20 Hz), and low gamma (30-40 Hz) oscillations. All oscillations were stronger in awake animals. While the power of delta, theta, and low beta oscillations increased with depth, the power of low gamma was more constant throughout L1. These findings identify L1 as an important coordination hub for the dynamic binding process of neurons mediated by oscillations.

source:https://creativecommons.org/licenses/by/4.0/

source:https://www.eneuro.org/content/7/3/ENEURO.0274-19.2020

収録刊行物

  • eneuro

    eneuro 7 (3), ENEURO.0274-19.2020-, 2020-04-15

    Society for Neuroscience

詳細情報

  • CRID
    1050285700328586624
  • NII論文ID
    120006886505
  • ISSN
    23732822
  • Web Site
    http://id.nii.ac.jp/1394/00001501/
  • 本文言語コード
    en
  • 資料種別
    journal article
  • データソース種別
    • IRDB
    • CiNii Articles

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