Multiomics Investigation Revealing the Characteristics of HIV-1-Infected Cells InVivo

• 麻生, 啓文

• 長岡, 峻平

• 川上, 英良

• 伊東, 潤平

• 中岡, 慎治

• 芦崎, 晃一

• 城口, 克之

• 鈴木, 穣

• 佐藤, 賢文

• 小柳, 義夫

• 佐藤, 佳

• Koyanagi, Yoshio

• Sato, Kei

抄録

For eradication of HIV-1 infection, it is important to elucidate the detailed features and heterogeneity of HIV-1-infected cells in vivo. To reveal multiple characteristics of HIV-1-producing cells in vivo, we use a hematopoietic-stem-cell-transplanted humanized mouse model infected with GFP-encoding replication-competent HIV-1. We perform multiomics experiments using recently developed technology to identify the features of HIV-1-infected cells. Genome-wide HIV-1 integration-site analysis reveals that productive HIV-1 infection tends to occur in cells with viral integration into transcriptionally active genomic regions. Bulk transcriptome analysis reveals that a high level of viral mRNA is transcribed in HIV-1-infected cells. Moreover, single-cell transcriptome analysis shows the heterogeneity of HIV-1-infected cells, including CXCL13high cells and a subpopulation with low expression of interferon-stimulated genes, which can contribute to efficient viral spread in vivo. Our findings describe multiple characteristics of HIV-1-producing cells in vivo, which could provide clues for the development of an HIV-1 cure.

収録刊行物

• Cell Reports

  Cell Reports 32 (2), 2020-07-14

  Elsevier BV

キーワード

• HIV
• humanized mouse
• transcriptome
• genome
• multiomics

詳細情報

• CRID: 1050567175332640384
• NII論文ID: 120006888452
• ISSN: 22111247
• HANDLE: 2433/255120
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles