Self-Organized Synchronous Calcium Transients in a Cultured Human Neural Network Derived from Cerebral Organoids

• 坂口, 秀哉

• 尾崎, 由季

• 芦田, 知佳

• 松原, 孝宜

• 髙橋, 淳

• Kihara, Shunsuke

• Takahashi, Jun

抄録

The cerebrum is a major center for brain function, and its activity is derived from the assembly of activated cells in neural networks. It is currently difficult to study complex human cerebral neuronal network activity. Here, using cerebral organoids, we report self-organized and complex human neural network activities that include synchronized and non-synchronized patterns. Self-organized neuronal network formation was observed following a dissociation culture of human embryonic stem cell-derived cerebral organoids. The spontaneous individual and synchronized activity of the network was measured via calcium imaging, and subsequent analysis enabled the examination of detailed cell activity patterns, providing simultaneous raster plots, cluster analyses, and cell distribution data. Finally, we demonstrated the feasibility of our system to assess drug-inducible dynamic changes of the network activity. The comprehensive functional analysis of human neuronal networks using this system may offer a powerful tool to access human brain function.

収録刊行物

• Stem Cell Reports

  Stem Cell Reports 13 (3), 458-473, 2019-09-10

  Elsevier BV

キーワード

• cerebral organoid
• human pluripotent stem cell
• three-dimensional imaging
• calcium imaging
• neural network activity

詳細情報

• CRID: 1050567175332674560
• NII論文ID: 120006888502
• ISSN: 22136711
• HANDLE: 2433/255246
• 本文言語コード: ja
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles
  • KAKEN