Characterization of a gene regulatory network underlying astringency loss in persimmon fruit

• 尾上, 典之

• 河野, 淳

• 佐藤, 明彦

Abstract

Main conclusion Transcriptome analysis of a persimmon population segregating for an astringency trait in fruit suggested central roles for a limited number of transcriptional regulators in the loss of proanthocyanidin accumulation.Persimmon (Diospyros kaki; 2n = 6x = 90) accumulates a large amount of proanthocyanidins (PAs) in its fruit, resulting in an astringent taste. Persimmon cultivars are classified into four types based on the nature of astringency loss and the amount of PAs at maturity. Pollination-constant and non-astringent (PCNA)-type cultivars stop accumulating PAs in the early stages of fruit development and their fruit can be consumed when still firm without the need for artificial deastringency treatments. While the PCNA trait has been shown to be conferred by a recessive allele at a single locus (ASTRINGENCY; AST), the exact genetic determinant remains unidentified. Here, we conducted transcriptome analyses to elucidate the regulatory mechanism underlying this trait using developing fruits of a F1 population segregating for the PCNA trait. Comparisons of the transcriptomes of PCNA and non-PCNA individuals and hierarchical clustering revealed that genes related to the flavonoid pathway and to abiotic stress responses involving light stimulation wereexpressed coordinately with PA accumulation. Furthermore, coexpression network analyses suggested that three putative transcription factors were central to the PA regulatory network and that at least DkMYB4 and/or DkMYC1, which have been reported to form a protein complex with each other for PA regulation, may have a central role in the differential expression of PA biosynthetic pathway genes between PCNA and non-PCNA.

source:SC30201804130055

source:NARO成果DBa

source:This is a post-peer-review, pre-copyedit version of an article published in [Planta].

source:The final authenticated version is available online at: https://doi.org/10.1007/s00425-017-2819-0

source:author manuscript

source:このアーカイブは著者版です。出版社版ではありません。引用の際には出版社版をご利用ください。

source:This is not the published version. Please cite only the published version.

source:https://doi.org/10.1007/s00425-017-2819-0

Journal

• Planta = Planta

  Planta = Planta 247 (3), 733-743, 2017-09-29

  Springer Nature

Keywords

• Abiotic stress
• Coexpression network analysis
• Diospyros kaki
• Fruit transcriptome
• Proanthocyanidins

Details

• CRID: 1050567903451220480
• NII Article ID: 120006892318
• ISSN: 00320935
• Web Site: http://id.nii.ac.jp/1578/00002562/
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • CiNii Articles