Trans-omics Impact of Thymoproteasome in Cortical Thymic Epithelial Cells

• Ohigashi, Izumi

• Tanaka, Yu

• Kondo, Kenta

• Fujimori, Sayumi

• Kondo, Hiroyuki

• Palin, Amy C.

• Hoffmann, Victoria

• Kozai, Mina

• Matsushita, Yosuke

• Uda, Shinsuke

• Motosugi, Ryo

• Hamazaki, Jun

• Kubota, Hiroyuki

• Murata, Shigeo

• Tanaka, Keiji

• Katagiri, Toyomasa

• Kosako, Hidetaka

• Takahama, Yousuke

抄録

The thymic function to produce self-protective and self-tolerant T cells is chiefly mediated by cortical thymic epithelial cells (cTECs) and medullary TECs (mTECs). Recent studies including single-cell transcriptomic analyses have highlighted a rich diversity in functional mTEC subpopulations. Because of their limited cellularity, however, the biochemical characterization of TECs, including the proteomic profiling of cTECs and mTECs, has remained unestablished. Utilizing genetically modified mice that carry enlarged but functional thymuses, here we show a combination of proteomic and transcriptomic profiles for cTECs and mTECs, which identified signature molecules that characterize a developmental and functional contrast between cTECs and mTECs. Our results reveal a highly specific impact of the thymoproteasome on proteasome subunit composition in cTECs and provide an integrated trans-omics platform for further exploration of thymus biology.

収録刊行物

• Cell Reports

  Cell Reports 29 (9), 2901-2916, 2019-11-26

  Elsevier

キーワード

• General Biochemistry, Genetics and Molecular Biology

詳細情報

• CRID: 1051130853409273472
• NII論文ID: 120006894616
• ISSN: 22111247
• DOI: 10.1016/j.celrep.2019.10.079
• Web Site: http://repo.lib.tokushima-u.ac.jp/115049
• Web Site: https://api.elsevier.com/content/article/PII:S2211124719314019?httpAccept=text/xml
• Web Site: https://api.elsevier.com/content/article/PII:S2211124719314019?httpAccept=text/plain
• 本文言語コード: en
• 資料種別: journal article
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