Scan-Less, Kilo-Pixel, Line-Field Confocal Phase Imaging with Spectrally Encoded Dual-Comb Microscopy

• Hase, Eiji

• Minamikawa, Takeo

• Miyamoto, Shuji

• Ichikawa, Ryuji

• Hiseh, Yi-Da

• Mizutani, Yasuhiro

• Iwata, Tetsuo

• Yamamoto, Hirotsugu

• Yasui, Takeshi

抄録

Confocal laser microscopy (CLM) is a powerful tool in life science research and industrial inspection, and its image acquisition rate is boosted by scan-less imaging techniques. However, the optical-intensity-based image contrast in CLM makes it difficult to visualize transparent non-fluorescent objects or reflective objects with nanometer unevenness. In this paper, we introduce an optical frequency comb (OFC) to scan-less CLM to give the optical-phase-based image contrast. One-dimensional (1D) image pixels of a sample are separately encoded onto OFC modes via 1D spectral encoding by using OFC as an optical carrier of amplitude and phase with a vast number of discrete frequency channels. Then, line-field confocal information of amplitude and phase are decoded from a mode-resolved OFC amplitude and phase spectra obtained by dual-comb spectroscopy. The proposed confocal phase imaging will further expand the application fields of CLM.

収録刊行物

• IEEE Journal of Selected Topics in Quantum Electronics

  IEEE Journal of Selected Topics in Quantum Electronics 25 (1), 6801408-, 2018-11-09

  IEEE

キーワード

• Biomedical imaging
• Optical microscopy
• Optical interferometry

詳細情報

• CRID: 1050567903550163200
• NII論文ID: 120006937692
• NII書誌ID: AA11036333
• ISSN: 1077260X
• ISSN: 15584542
• Web Site: http://repo.lib.tokushima-u.ac.jp/113637
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles