A four-dimensional organoid system to visualize cancer cell vascular invasion

• Yanagisawa, Kiminori

• Konno, Masamitsu

• Liu, Hao

• Irie, Shinji

• Mizushima, Tsunekazu

• Mori, Masaki

• Doki, Yuichiro

• Eguchi, Hidetoshi

• Matsusaki, Michiya

• Ishii, Hideshi

Abstract

Yanagisawa, K.; Konno, M.; Liu, H.; Irie, S.; Mizushima, T.; Mori, M.; Doki, Y.; Eguchi, H.; Matsusaki, M.; Ishii, H. A Four-Dimensional Organoid System to Visualize Cancer Cell Vascular Invasion. Biology 2020, 9, 361.

Vascular invasion of cancer is a critical step in cancer progression, but no drug has been developed to inhibit vascular invasion. To achieve the eradication of cancer metastasis, elucidation of the mechanism for vascular invasion and the development of innovative treatment methods are required. Here, a simple and reproducible vascular invasion model is established using a vascular organoid culture in a fibrin gel with collagen microfibers. Using this model, it was possible to observe and evaluate the cell dynamics and histological positional relationship of invasive cancer cells in four dimensions. Cancer-derived exosomes promoted the vascular invasion of cancer cells and loosened tight junctions in the vascular endothelium. As a new evaluation method, research using this vascular invasion mimic model will be advanced, and applications to the evaluation of the vascular invasion suppression e_ect of a drug are expected.

Journal

• Biology

  Biology 9 (11), 361-, 2020-11

  MDPI

Keywords

• 4D culture
• Exosome
• Mimic model
• Tight junction
• Vascular invasion
• Vascular organoid

Details

• CRID: 1050862643878372992
• NII Article ID: 120006952170
• DOI: 10.3390/biology9110361
• ISSN: 20797737
• HANDLE: 11094/78290
• Web Site: https://www.mdpi.com/2079-7737/9/11/361/pdf
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • Crossref
  • CiNii Articles
  • KAKEN