Elucidation of molecular functions of human tumor suppressor protein 101F6 by reconstitution into phospholipid bilayer nanodiscs

• Behery, Mohammed El

• Asada, Akikazu

• Takeuchi, Fusako

• Kimura, Tetsunari

• Chatani, Eri

• Tsubaki, Motonari

Abstract

A candidate human tumor suppressor gene 101F6 product was expressed successfully in Pichia pastoris yeast cells. The purified 101F6 protein was successfully incorporated into phospholipid bilayer nanodiscs with different sizes by employing two reconstitution methods; self-assembly and reconstitution into the preformed empty nanodisc. The reconstituted 101F6 protein could be reduced with ascorbate quickly and was very stable even at ambient temperatures.

Journal

• journal of the Kapisanang Kimika ng Pilipinas

  journal of the Kapisanang Kimika ng Pilipinas 30 (1), 1-3, 2019-06-03

  Chemical Society of the Philippines

Keywords

• cytochrome b561
• nanodisc
• ascorbate
• membrane protein
• electron transfer
• apoptosis

Details

• CRID: 1050012570391855488
• NII Article ID: 120006952747
• ISSN: 25080911
• ISSN: 01152130
• HANDLE: 20.500.14094/90007775
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • CiNii Articles