Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis

• Ueda, Jun

• Harada, Akihito

• Urahama, Takashi

• Machida, Shinichi

• Maehara, Kazumitsu

• Hada, Masashi

• Makino, Yoshinori

• Nogami, Jumpei

• Horikoshi, Naoki

• Osakabe, Akihisa

• Taguchi, Hiroyuki

• Tanaka, Hiroki

• Tachiwana, Hiroaki

• Yao, Tatsuma

• Yamada, Minami

• Iwamoto, Takashi

• Isotani, Ayako

• Ikawa, Masahito

• Tachibana, Taro

• Okada, Yuki

• Kimura, Hiroshi

• Ohkawa, Yasuyuki

• Kurumizaka, Hitoshi

• Yamagata, Kazuo

Abstract

Jun Ueda, Akihito Harada, Takashi Urahama, Shinichi Machida, Kazumitsu Maehara, Masashi Hada, Yoshinori Makino, Jumpei Nogami, Naoki Horikoshi, Akihisa Osakabe, Hiroyuki Taguchi, Hiroki Tanaka, Hiroaki Tachiwana, Tatsuma Yao, Minami Yamada, Takashi Iwamoto, Ayako Isotani, Masahito Ikawa, Taro Tachibana, Yuki Okada, Hiroshi Kimura, Yasuyuki Ohkawa, Hitoshi Kurumizaka, Kazuo Yamagata, Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis, Cell Reports, Volume 18, Issue 3, 2017, Pages 593-600, ISSN 2211-1247, https://doi.org/10.1016/j.celrep.2016.12.065.

Cellular differentiation is associated with dynamic chromatin remodeling in establishing a cell-type-specific epigenomic landscape. Here, we find that mouse testis-specific and replication-dependent histone H3 variant H3t is essential for very early stages ofspermatogenesis. H3t gene deficiency leads to azoospermia because of the loss of haploid germ cells. When differentiating spermatogonia emerge in normal spermatogenesis, H3t appears and replaces the canonical H3 proteins. Structural and biochemical analyses reveal that H3t-containing nucleosomes are more flexible than the canonical nucleosomes. Thus, by incorporating H3t into the genome during spermatogonial differentiation, male germ cells are able to enter meiosis and beyond.

Journal

• Cell Reports

  Cell Reports 18 (3), 593-600, 2017-01-17

  Elsevier B.V.

Keywords

• chromatin
• crystal structure
• epigenetics
• histone variant
• meiosis
• nucleosome
• spermatogenesis
• spermatogonial differentiation
• spermatozoa
• testes

Details

• CRID: 1050299693924815232
• NII Article ID: 120006957416
• ISSN: 22111247
• HANDLE: 11094/78582
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • CiNii Articles