Label-free imaging by stimulated parametric emission microscopy reveals a difference in hemoglobin distribution between live and fixed erythrocytes

• Dang, Hieu M.

• Omura, Gen

• Umano, Toshiyuki

• Yamagiwa, Masatomo

• Kajiyama, Shin'ichiro

• Ozeki, Yasuyuki

• Itoh, Kazuyoshi

• Fukui, Kiichi

Abstract

Hieu M. Dang, Gen Omura, Toshiyuki Omano, Masatomo Yamagiwa, Shin'ichiro Kajiyama, Yasuyuki Ozeki, Kazuyoshi Itoh, and Kiichi Fukui "Label-free imaging by stimulated parametric emission microscopy reveals a difference in hemoglobin distribution between live and fixed erythrocytes," Journal of Biomedical Optics 14(4), 040506 (1 July 2009). https://doi.org/10.1117/1.3207151

We demonstrate that stimulated parametric emission (SPE) microscopy enables label-free, 3-D visualization of internal hemoglobin distribution of live mouse and chicken erythrocytes with high sensitivity. Change in hemoglobin distribution in chicken erythrocytes before and after ethanol fixation is clearly visualized. © 2009 Society of Photo-Optical Instrumentation Engineers.

Journal

• Journal of Biomedical Optics

  Journal of Biomedical Optics 14 (4), 040506-, 2009-07-01

  SPIE

Keywords

• hemoglobin
• nonlinear microscopy
• stimulated parametric emission
• three dimensional
• unstained

Details

• CRID: 1050581168901413120
• NII Article ID: 120006980200
• NII Book ID: AA11237889
• ISSN: 15602281
• ISSN: 10833668
• HANDLE: 11094/78972
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • CiNii Articles