Secreted factors from cultured dental pulp stem cells promoted neurite outgrowth of dorsal root ganglion neurons and ameliorated neural functions in streptozotocin‐induced diabetic mice

• Miura‐Yura, Emiri

• Tsunekawa, Shin

• Naruse, Keiko

• Nakamura, Nobuhisa

• Motegi, Mikio

• Nakai‐Shimoda, Hiromi

• Asano, Saeko

• Kato, Makoto

• Yamada, Yuichiro

• Izumoto‐Akita, Takako

• Yamamoto, Akihito

• Himeno, Tatsuhito

• Kondo, Masaki

• Kato, Yoshiro

• Nakamura, Jiro

• Kamiya, Hideki

書誌事項

タイトル別名

• Effect of SHED-CM on DPN

抄録

Aims/Introduction: Transplantation of stem cells promotes axonal regeneration and angiogenesis in a paracrine manner. In the present study, we examined whether the secreted factors in conditioned medium of stem cells from human exfoliated deciduous teeth (SHED‐CM) had beneficial effects on diabetic polyneuropathy in mice. Materials and Methods: Conditioned medium of stem cells from human exfoliated deciduous teeth was collected 48 h after culturing in serum‐free Dulbecco's modified Eagle's medium (DMEM), and separated into four fractions according to molecular weight. Dorsal root ganglion neurons from C57BL/6J mice were cultured with SHED‐CM or DMEM to evaluate the effect on neurite outgrowth. Streptozotocin‐induced diabetic mice were injected with 100 μL of SHED‐CM or DMEM into the unilateral hindlimb muscles twice a week over a period of 4 weeks. Peripheral nerve functions were evaluated by the plantar test, and motor and sensory nerve conduction velocities. Intraepidermal nerve fiber densities, capillary number‐to‐muscle fiber ratio, capillary blood flow and morphometry of sural nerves were also evaluated. Results: Conditioned medium of stem cells from human exfoliated deciduous teeth significantly promoted neurite outgrowth of dorsal root ganglion neurons compared with DMEM. Among four fractions of SHED‐CM, the only fraction of <6 kDa promoted the neurite outgrowth of dorsal root ganglion neurons. In addition, SHED‐CM significantly prevented decline in sensory nerve conduction velocities compared with DMEM in diabetic mice. Although SHED‐CM did not improve intraepidermal nerve fiber densities or morphometry of sural nerves, SHED‐CM ameliorated the capillary number‐to‐muscle fiber ratio and capillary blood flow. Conclusions: These results suggested that SHED‐CM might have a therapeutic effect on diabetic polyneuropathy through promoting neurite outgrowth, and the increase in capillaries might contribute to the improvement of neural function.

収録刊行物

• Journal of Diabetes Investigation

  Journal of Diabetes Investigation 11 (1), 28-38, 2019-05-29

  Asian Association for the Study of Diabetes|John Wiley & Sons

キーワード

• Conditioned medium
• Diabetic polyneuropathy
• Stem cells from human exfoliated deciduous teeth

詳細情報

• CRID: 1050850247203472000
• NII論文ID: 120006980549
• NII書誌ID: AA12488319
• ISSN: 20401124
• ISSN: 20401116
• DOI: 10.1111/jdi.13085
• Web Site: http://repo.lib.tokushima-u.ac.jp/115502
• Web Site: https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjdi.13085
• Web Site: https://onlinelibrary.wiley.com/doi/pdf/10.1111/jdi.13085
• Web Site: https://onlinelibrary.wiley.com/doi/full-xml/10.1111/jdi.13085
• 本文言語コード: en
• 資料種別: journal article
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