Ultrasensitive human prion detection in cerebrospinal fluid by real-time quaking-induced conversion.

• Atarashi, Ryuichiro

• Satoh, Katsuya

• Sano, Kazunori

• Fuse, Takayuki

• Yamaguchi, Naohiro

• Ishibashi, Daisuke

• Matsubara, Takehiro

• Nakagaki, Takehiro

• Yamanaka, Hitoki

• Shirabe, Susumu

• Yamada, Masahito

• Mizusawa, Hidehiro

• Kitamoto, Tetsuyuki

• Klug, Genevieve

• McGlade, Amelia

• Collins, Steven J

• Nishida, Noriyuki

Abstract

The development of technologies for the in vitro amplification of abnormal conformations of prion protein (PrP(Sc)) has generated the potential for sensitive detection of prions. Here we developed a new PrP(Sc) amplification assay, called real-time quaking-induced conversion (RT-QUIC), which allows the detection of ≥1 fg of PrP(Sc) in diluted Creutzfeldt-Jakob disease (CJD) brain homogenate. Moreover, we assessed the technique first in a series of Japanese subjects and then in a blind study of 30 cerebrospinal fluid specimens from Australia, which achieved greater than 80% sensitivity and 100% specificity. These findings indicate the promising enhanced diagnostic capacity of RT-QUIC in the antemortem evaluation of suspected CJD.

Nature Medicine, 17(2), pp.175-178; 2011

Journal

• Nature Medicine

  Nature Medicine 17 (2), 175-178, 2011-02

  Nature Publishing Group

Details

• CRID: 1050287297274658944
• NII Article ID: 120006984771
• NII Book ID: AA11029600
• ISSN: 10788956
• ISSN: 1546170X
• HANDLE: 10069/25642
• Web Site: https://nagasaki-u.repo.nii.ac.jp/records/14954
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • CiNii Articles
  • KAKEN