CD8+ T cell activation by murine erythroblasts infected with malaria parasites

• Imai, Takashi

• Ishida, Hidekazu

• Suzue, Kazutomo

• Hirai, Makoto

• Taniguchi, Tomoyo

• Okada, Hiroko

• Suzuki, Tomohisa

• Shimokawa, Chikako

• Hisaeda, Hajime

Abstract

Recent studies show that some human malaria parasite species Plasmodium falciparum and P. vivax parasitize erythroblasts; however, the biological and clinical significance of this is unclear. To investigate further, we generated a rodent malaria parasite (P. yoelii 17XNL) expressing GFP-ovalbumin (OVA). Its infectivity to erythroblasts was confirmed, and parasitized erythroblasts were capable of initiating malaria infections. Experiments showed that MHC class I molecules were highly expressed on parasitized erythroblasts. As CD8+ T cells recognize MHC class I and peptide complexes on target cells, and are involved in protection or pathology against malaria, we examined whether erythroblasts are targeted by CD8+ T cells. Purified non-parasitized erythroblasts pulsed with OVA peptides were recognized by OVA-specific CD8 + T cells. Crucially, parasitized erythroblasts isolated from GFP-OVA-, but not GFP-infected-mice, activated OT-I CD8+ T cells, indicating that CD8+ T cells recognize parasitized erythroblasts in an antigen-specific manner.

Scientific Reports, 3, 1572; 2013

Journal

• Scientific Reports

  Scientific Reports 3 1572-, 2013-03-28

  Nature Publishing Group

Keywords

• Multidisciplinary

Details

• CRID: 1050850247205364992
• NII Article ID: 120006985954
• ISSN: 20452322
• DOI: 10.1038/srep01572
• HANDLE: 10069/32141
• Web Site: https://nagasaki-u.repo.nii.ac.jp/records/7298
• Web Site: https://www.nature.com/articles/srep01572
• Web Site: https://www.nature.com/articles/srep01572.pdf
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • Crossref
  • CiNii Articles
  • KAKEN