Sialic acid-dependent attachment of mucins from three mouse strains to Entamoeba histolytica

• Kato, Kentaro

• Takegawa, Yasuhiro

• Ralston, Katherine S.

• Gilchrist, Carol A.

• Hamano, Shinjiro

• Petri, William A.

• Shinohara, Yasuro

抄録

Mouse strain-specific differences in the carbohydrate composition of intestinal mucins were hypothesized to account for strain-dependent susceptibility to Entamoeba histolytica. To test this hypothesis, intestinal mucins from susceptible and resistant inbred strains of mice were analyzed for their O-glycan content and for their ability to inhibit amoebic adherence to (GalNAc)12-27-HSA neo-glycoproteins. The results showed that the colorectal mucin O-glycan of susceptible CBA mice was lower in sialic acid content than that of resistant C57BL/6 and BALB/c mice. Mucins from CBA mice were more potent inhibitors of E. histolytica adherence to neo-glycoproteins than were mucins from C57BL/6 or BALB/c mice. Consistent with the role of terminal Gal/GalNAc as a receptor for amoebic adherence, sialidase treatment of C57BL/6 and BALB/c colorectal mucins increased their ability to inhibit E. histolytica adherence to the neo-glycoproteins. These results provide evidence of mouse strain-specific differences in the sialic acids content of mucin O-glycans. These dissimilarities likely contribute to the differential susceptibility of the three mouse strains to E. histolytica infection.

Biochemical and Biophysical Research Communications, 436(2), pp.252-258; 2013

収録刊行物

• Biochemical and Biophysical Research Communications

  Biochemical and Biophysical Research Communications 436 (2), 252-258, 2013-06-28

  Academic Press Inc.

キーワード

• Entamoeba histolytica
• Lectin
• Mucin
• O-glycan
• Sialic acid

詳細情報

• CRID: 1050005822298568704
• NII論文ID: 120006986202
• ISSN: 0006291X
• DOI: 10.1016/j.bbrc.2013.05.085
• HANDLE: 10069/33394
• Web Site: https://nagasaki-u.repo.nii.ac.jp/records/6439
• Web Site: https://api.elsevier.com/content/article/PII:S0006291X13008929?httpAccept=text/xml
• Web Site: https://api.elsevier.com/content/article/PII:S0006291X13008929?httpAccept=text/plain
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
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