Prion-Seeding Activity Is widely Distributed in Tissues of Sporadic Creutzfeldt-Jakob Disease Patients

• Takatsuki, Hanae

• Fuse, Takayuki

• Nakagaki, Takehiro

• Mori, Tsuyoshi

• Mihara, Ban

• Takao, Masaki

• Iwasaki, Yasushi

• Yoshida, Mari

• Murayama, Shigeo

• Atarashi, Ryuichiro

• Nishida, Noriyuki

• Satoh, Katsuya

Abstract

Human prion diseases are neurodegenerative disorders caused by abnormally folded prion proteins in the central nervous system. These proteins can be detected using the quaking-induced conversion assay. Compared with other bioassays, this assay is extremely sensitive and was used in the present study to determine prion distribution in sporadic Creutzfeldt-Jakob disease patients at autopsy. Although infectivity of the sporadic form is thought to be restricted within the central nervous system, results showed that prion-seeding activities reach 106/g from a 50% seeding dose in non-neuronal tissues, suggesting that prion-seeding activity exists in non-neural organs, and we suggested that non-neural tissues of 106/g SD50 did not exist the infectivity.

EBioMedicine, 12, pp.150-155; 2016

Journal

• EBioMedicine

  EBioMedicine 12 150-155, 2016-10

  Elsevier B.V.

Keywords

• Creutzfeldt-Jakob disease
• Non-neural tissue
• Prion
• Prion-seeding activity
• SD50

Details

• CRID: 1050850247228979584
• NII Article ID: 120006987618
• ISSN: 23523964
• HANDLE: 10069/37342
• Web Site: https://nagasaki-u.repo.nii.ac.jp/records/2144
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • CiNii Articles