Spontaneous Development of Dental Dysplasia in Aged Parp-1 Knockout Mice

• Fujihara, Hisako

• Nozaki, Tadashige

• Tsutsumi, Masahiro

• Isumi, Mayu

• Shimoda, Shinji

• Hamada, Yoshiki

• Masutani, Mitsuko

抄録

Poly(ADP-ribose) polymerase (Parp)-1 catalyzes polyADP-ribosylation using NAD+ and is involved in the DNA damage response, genome stability, and transcription. In this study, we demonstrated that aged Parp-1-/- mouse incisors showed more frequent dental dysplasia in both ICR/129Sv mixed background and C57BL/6 strain compared to aged Parp-1+/+ incisors, suggesting that Parp-1 deficiency could be involved in development of dental dysplasia at an advanced age. Computed tomography images confirmed that dental dysplasia was observed at significantly higher incidences in Parp-1-/- mice. The relative calcification levels of Parp-1-/- incisors were higher in both enamel and dentin (p < 0.05). Immunohistochemical analysis revealed (1) Parp-1 positivity in ameloblasts and odontoblasts in Parp-1+/+ incisor, (2) weaker dentin sialoprotein positivity in dentin of Parp-1-/- incisor, and (3) bone sialoprotein positivity in dentin of Parp-1-/- incisor, suggesting ectopic osteogenic formation in dentin of Parp-1-/- incisor. These results indicate that Parp-1 deficiency promotes odontogenic failure in incisors at an advanced age. Parp-1 deficiency did not affect dentinogenesis during the development of mice, suggesting that Parp-1 is not essential in dentinogenesis during development but is possibly involved in the regulation of continuous dentinogenesis in the incisors at an advanced age.

Cells, 8(10), art.no.1157; 2020

収録刊行物

• Cells

  Cells 8 (10), 1157-, 2019-09-27

  MDPI

キーワード

• poly(ADP-ribose) polymerase-1
• aged mice
• knockout mice
• dental dysplasia

詳細情報

• CRID: 1050287297263975680
• NII論文ID: 120006988121
• ISSN: 20734409
• DOI: 10.3390/cells8101157
• HANDLE: 10069/40145
• Web Site: https://nagasaki-u.repo.nii.ac.jp/records/951
• Web Site: https://www.mdpi.com/2073-4409/8/10/1157/pdf
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
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  • Crossref
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