Stimulated raman scattering microscope with shot noise limited sensitivity using subharmonically synchronized laser pulses

• Ozeki, Yasuyuki

• Kitagawa, Yuma

• Sumimura, Kazuhiko

• Nishizawa, Norihiko

• Umemura, Wataru

• Kajiyama, Shin'ichiro

• Fukui, Kiichi

• Itoh, Kazuyoshi

Abstract

Ozeki Y., Kitagawa Y., Sumimura K., et al. Stimulated raman scattering microscope with shot noise limited sensitivity using subharmonically synchronized laser pulses. Optics Express, 18, 13, 13708. https://doi.org/10.1364/OE.18.013708.

We propose and demonstrate the use of subharmonically synchronized laser pulses for low-noise lock-in detection in stimulated Raman scattering (SRS) microscopy. In the experiment, Yb-fiber laser pulses at a repetition rate of 38 MHz are successfully synchronized to Ti:sapphire laser pulses at a repetition rate of 76 MHz with a jitter of <8 fs by a two-photon detector and an intra-cavity electro-optic modulator. By using these pulses, high-frequency lock-in detection of SRS signal is accomplished without high-speed optical modulation. The noise level of the lock-in signal is found to be higher than the shot noise limit only by 1.6 dB. We also demonstrate high-contrast, 3D imaging of unlabeled living cells.

Journal

• Optics Express

  Optics Express 18 (13), 13708-13719, 2010-06-21

  Optical Society of American

Details

• CRID: 1050862643878102144
• NII Article ID: 120006994999
• ISSN: 10944087
• HANDLE: 11094/79041
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • CiNii Articles