Mutations in hik26 and slr1916 lead to high-light stress tolerance in Synechocystis sp. PCC6803

• Yoshikawa, Katsunori

• Ogawa, Kenichi

• Toya, Yoshihiro

• 秋本, 誠志

• Matsuda, Fumio

• Shimizu, Hiroshi

抄録

Increased tolerance to light stress in cyanobacteria is a desirable feature for their applications. Here, we obtained a high light tolerant (Tol) strain of Synechocystis sp. PCC6803 through an adaptive laboratory evolution, in which the cells were repeatedly sub-cultured for 52 days under high light stress conditions (7000 to 9000 μmol m−2 s−1). Although the growth of the parental strain almost stopped when exposed to 9000 μmol m−2 s−1, no growth inhibition was observed in the Tol strain. Excitation-energy flow was affected because of photosystem II damage in the parental strain under high light conditions, whereas the damage was alleviated and normal energy flow was maintained in the Tol strain. The transcriptome data indicated an increase in isiA expression in the Tol strain under high light conditions. Whole genome sequence analysis and reverse engineering revealed two mutations in hik26 and slr1916 involved in high light stress tolerance in the Tol strain.

収録刊行物

• communications biology

  communications biology 4 343-, 2021-03-16

  Nature Research

キーワード

• General Agricultural and Biological Sciences
• General Biochemistry, Genetics and Molecular Biology
• Medicine (miscellaneous)

詳細情報

• CRID: 1050012570391820928
• NII論文ID: 120007028956
• DOI: 10.1038/s42003-021-01875-y
• ISSN: 23993642
• HANDLE: 20.500.14094/90008236
• Web Site: https://www.nature.com/articles/s42003-021-01875-y.pdf
• Web Site: https://www.nature.com/articles/s42003-021-01875-y
• 本文言語コード: en
• 資料種別: journal article
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