Multiple gene substitution by Target-AID base-editing technology in tomato

• Hunziker, Johan

• 西田, 敬二

• 近藤, 昭彦

• Kishimoto, Sanae

• Ariizumi, Tohru

• Ezura, Hiroshi

抄録

The use of Target activation-induced cytidine deaminase (Target-AID) base-editing technology with the CRISPR-Cas 9 system fused with activation-induced cytidine deaminase (AID) resulted in the substitution of a cytidine with a thymine. In previous experiments focusing on a single target gene, this system has been reported to work in several plant species, including tomato (Solanum lycopersicum L.). In this research, we used Target-AID technology to target multiple genes related to carotenoid accumulation in tomato. We selected 3 genes, SlDDB1, SlDET1 and SlCYC-B, for their roles in carotenoid accumulation. Among 12 edited T0 lines, we obtained 10 independent T0 lines carrying nucleotide substitutions in the three targeted genes, with several allelic versions for each targeted gene. The two edited lines showed significant differences in carotenoid accumulation. These results demonstrate that Target-AID technology is a highly efficient tool for targeting multiple genes with several allelic versions.

収録刊行物

• scientific reports

  scientific reports 10 20471-, 2020-11-24

  Nature Research

キーワード

• Multidisciplinary

詳細情報

• CRID: 1050012570391941760
• NII論文ID: 120007028992
• DOI: 10.1038/s41598-020-77379-2
• ISSN: 20452322
• HANDLE: 20.500.14094/90008205
• HANDLE: 2241/0002004679
• Web Site: https://tsukuba.repo.nii.ac.jp/records/2004679
• Web Site: https://www.nature.com/articles/s41598-020-77379-2.pdf
• Web Site: https://www.nature.com/articles/s41598-020-77379-2
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
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