Modeling SARS-CoV-2 infection and its individual differences with ACE2-expressing human iPS cells

• Sano, Emi

  Center for iPS Cell Research and Application (CiRA), Kyoto University

• Deguchi, Sayaka

  Center for iPS Cell Research and Application (CiRA), Kyoto University

• Sakamoto, Ayaka

  Center for iPS Cell Research and Application (CiRA), Kyoto University

• Mimura, Natsumi

  Center for iPS Cell Research and Application (CiRA), Kyoto University

• Hirabayashi, Ai

  CREST, Japan Science and Technology Agency (JST); Institute for the Advanced Study of Human Biology (WPI-ASHBi), Kyoto University

• Muramoto, Yukiko

  CREST, Japan Science and Technology Agency (JST); Institute for the Advanced Study of Human Biology (WPI-ASHBi), Kyoto University

• Noda, Takeshi

  CREST, Japan Science and Technology Agency (JST); Institute for the Advanced Study of Human Biology (WPI-ASHBi), Kyoto University

• Yamamoto, Takuya

  Center for iPS Cell Research and Application (CiRA), Kyoto University; Institute for the Advanced Study of Human Biology (WPI-ASHBi), Kyoto University; Medical-risk Avoidance Based on iPS Cells Team, RIKEN Center for Advanced Intelligence Project (AIP); AMED-CREST, Japan Agency for Medical Research and Development (AMED)

• Takayama, Kazuo

  Center for iPS Cell Research and Application (CiRA), Kyoto University

Abstract

Genetic differences are a primary reason for differences in the susceptibility and severity of COVID-19. As induced pluripotent stem (iPS) cells maintain the genetic information of the donor, they can be used to model individual differences in SARS-CoV-2 infection in vitro. We found that human iPS cells expressing the SARS-CoV-2 receptor angiotensin-converting enzyme 2 (ACE2) (ACE2-iPS cells) can be infected w SARS-CoV-2. In infected ACE2-iPS cells, the expression of SARS-CoV-2 nucleocapsid protein, budding of viral particles, and production of progeny virus, double membrane spherules, and double-membrane vesicles were confirmed. We performed SARS-CoV-2 infection experiments on ACE2-iPS/ embryonic stem (ES) cells from eight individuals. Male iPS/ES cells were more capable of producing the virus compared with female iPS/ES cells. These findings suggest that ACE2-iPS cells can not only reproduce individual differences in SARS-CoV-2 infection in vitro but also are a useful resource to clarify the causes of individual differences in COVID-19 due to genetic differences.

Stem cells show gender differences in COVID-19 risk. 京都大学プレスリリース. 2021-04-19.

Journal

• iScience

  iScience 24 (5), 102428-, 2021-05

  Elsevier BV

Keywords

• Health Sciences
• Virology
• Cell Biology

Details

• CRID: 1050850932905018368
• NII Article ID: 120007032353
• DOI: 10.1016/j.isci.2021.102428
• HANDLE: 2433/262724
• ISSN: 25890042
• Web Site: https://api.elsevier.com/content/article/PII:S2589004221003965?httpAccept=text/xml
• Web Site: https://api.elsevier.com/content/article/PII:S2589004221003965?httpAccept=text/plain
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • Crossref
  • CiNii Articles
  • KAKEN