Capturing structural changes of the S₁ to S₂ transition of photosystem II using time-resolved serial femtosecond crystallography

• Li, Hongjie

• Nakajima, Yoshiki

• Nomura, Takashi

• Sugahara, Michihiro

• Yonekura, Shinichiro

• Chan, Siu Kit

• Nakane, Takanori

• Yamane, Takahiro

• Umena, Yasufumi

• Suzuki, Mamoru

• Masuda, Tetsuya

• Motomura, Taiki

• Naitow, Hisashi

• Matsuura, Yoshinori

• Kimura, Tetsunari

• Tono, Kensuke

• Owada, Shigeki

• Joti, Yasumasa

• Tanaka, Rie

• Nango, Eriko

• Akita, Fusamichi

• Kubo, Minoru

• Iwata, So

• Shen, Jian-Ren

• Suga, Michihiro

書誌事項

タイトル別名

• Capturing structural changes of the S-1 to S-2 transition of photosystem II using time-resolved serial femtosecond crystallography

抄録

Photosystem II (PSII) catalyzes light-induced water oxidation through an S-i-state cycle, leading to the generation of di-oxygen, protons and electrons. Pumpprobe time-resolved serial femtosecond crystallography (TR-SFX) has been used to capture structural dynamics of light-sensitive proteins. In this approach, it is crucial to avoid light contamination in the samples when analyzing a particular reaction intermediate. Here, a method for determining a condition that avoids light contamination of the PSII microcrystals while minimizing sample consumption in TR-SFX is described. By swapping the pump and probe pulses with a very short delay between them, the structural changes that occur during the S-1-to-S-2 transition were examined and a boundary of the excitation region was accurately determined. With the sample flow rate and concomitant illumination conditions determined, the S-2-state structure of PSII could be analyzed at room temperature, revealing the structural changes that occur during the S-1-to-S-2 transition at ambient temperature. Though the structure of the manganese cluster was similar to previous studies, the behaviors of the water molecules in the two channels (O1 and O4 channels) were found to be different. By comparing with the previous studies performed at low temperature or with a different delay time, the possible channels for water inlet and structural changes important for the water-splitting reaction were revealed.

収録刊行物

• IUCrJ

  IUCrJ 8 431-443, 2021-05

  Int Union Crystallography

キーワード

• time-resolved serial crystallography
• X-ray free-electron lasers
• membrane proteins
• photosystem II
• serial crystallography
• molecular movies
• protein structures

詳細情報

• CRID: 1050288137385343616
• NII論文ID: 120007037379
• ISSN: 20522525
• DOI: 10.1107/s2052252521002177
• Web Site: https://ousar.lib.okayama-u.ac.jp/61958
• Web Site: http://journals.iucr.org/m/issues/2021/03/00/zf5015/zf5015.pdf
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
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