Determination of acid dissociation constants of flavin analogues by capillary zone electrophoresis

• Tanikami, Yuki

• Tagami, Takuma

• Sakamoto, Mayu

• Arakawa, Yukihiro

• Mizuguchi, Hitoshi

• Imada, Yasushi

• Takayanagi, Toshio

Abstract

Acid dissociation constants (pKa) of 9 kinds of flavin analogues as molecular catalyst candidates were determined by CZE. Although some of the analogues are instable and degradable under the light-exposure or in alkaline aqueous solutions, the effective electrophoretic mobility of the flavin analogue of interest has been measured with the residual substance. The pKa values of the flavin analogues were analyzed through the changes in the effective electrophoretic mobility with varying pH of the separation buffer. One or two steps pKa values were determined by the analysis. One of the degraded species from the flavin analogues, lumichrome, was also detected in the CZE analysis, and its pKa values were also determined. While coexisting impurities generated over the storage conditions were found in some analogues, the pKa values of the target analogues were successfully determined with the help of the CZE separations. A pressure-assisted CZE was utilized for the determination or the estimation of the pKa values of such analogues as possessing carboxylic acid moiety.

Journal

• Electrophoresis

  Electrophoresis 41 (15), 1316-1325, 2020-05-09

  WILEY-VCH

Keywords

• Acid dissociation constant
• Flavin analogues
• Instable substances
• Capillary zone electrophoresis

Details

• CRID: 1050569690505888640
• NII Article ID: 120007039999
• NII Book ID: AA00179882
• ISSN: 15222683
• ISSN: 01730835
• DOI: 10.1002/elps.202000066
• Web Site: http://repo.lib.tokushima-u.ac.jp/114776
• Web Site: https://onlinelibrary.wiley.com/doi/pdf/10.1002/elps.202000066
• Web Site: https://onlinelibrary.wiley.com/doi/full-xml/10.1002/elps.202000066
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • Crossref
  • CiNii Articles
  • KAKEN