Tracking the Fate of Endogenous Retrovirus Segregation in Wild and Domestic Cats

• Ngo, Minh Ha

• Arnal, MaríaCruz

• Sumi, Ryosuke

• Kawasaki, Junna

• Miyake, Ariko

• Grant, Chris K.

• Otoi, Takeshige

• de Luco, Daniel Fernández

• Nishigaki, Kazuo

• Viviana Simon

  editor

書誌事項

タイトル別名

• Fate of an Infectious ERV in Wild and Domestic Cats

抄録

Endogenous retroviruses (ERVs) of domestic cats (ERV-DCs) are one of the youngest feline ERV groups in domestic cats (Felis silvestris catus); some members are replication competent (ERV-DC10, ERV-DC18, and ERV-DC14), produce the antiretroviral soluble factor Refrex-1 (ERV-DC7 and ERV-DC16), or can generate recombinant feline leukemia virus (FeLV). Here, we investigated ERV-DC in European wildcats (Felis silvestris silvestris) and detected four loci: ERV-DC6, ERV-DC7, ERV-DC14, and ERV-DC16. ERV-DC14 was detected at a high frequency in European wildcats; however, it was replication defective due to a single G → A nucleotide substitution, resulting in an E148K substitution in the ERV-DC14 envelope (Env). This mutation results in a cleavage-defective Env that is not incorporated into viral particles. Introduction of the same mutation into feline and murine infectious gammaretroviruses resulted in a similar Env dysfunction. Interestingly, the same mutation was found in an FeLV isolate from naturally occurring thymic lymphoma and a mouse ERV, suggesting a common mechanism of virus inactivation. Refrex-1 was present in European wildcats; however, ERV-DC16, but not ERV-DC7, was unfixed in European wildcats. Thus, Refrex-1 has had an antiviral role throughout the evolution of the genus Felis, predating cat exposure to feline retroviruses. ERV-DC sequence diversity was present across wild and domestic cats but was locus dependent. In conclusion, ERVs have evolved species-specific phenotypes through the interplay between ERVs and their hosts. The mechanism of viral inactivation may be similar irrespective of the evolutionary history of retroviruses. The tracking of ancestral retroviruses can shed light on their roles in pathogenesis and host-virus evolution.

収録刊行物

• Journal of Virology

  Journal of Virology 93 (24), e01324-19-, 2019-11-26

  American Society for Microbiology

キーワード

• ERV-DC
• endogenous retrovirus
• Fv-4
• FeLV
• Felis
• MuLV
• domestic cat
• domestication
• evolution
• wildcat

詳細情報

• CRID: 1050569767908071680
• NII論文ID: 120007042421
• ISSN: 10985514
• ISSN: 0022538X
• DOI: 10.1128/jvi.01324-19
• Web Site: http://repo.lib.tokushima-u.ac.jp/116027
• Web Site: https://journals.asm.org/doi/pdf/10.1128/JVI.01324-19
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • Crossref
  • CiNii Articles
  • KAKEN