Two distinct motifs for Zic-r.a drive specific gene expression in two cell lineages

• 小田, いずみ

• Yu, Deli

• 佐藤, ゆたか

抄録

Zic-r.a, a maternal transcription factor, specifies posterior fate in ascidian embryos. However, its direct target, Tbx6-r.b, does not contain typical Zic-r.a-binding sites in its regulatory region. Using an in vitro selection assay, we found that Zic-r.a binds to sites dissimilar to the canonical motif, by which it activates Tbx6-r.b in a sub-lineage of muscle cells. These sites with non-canonical motifs have weak affinity for Zic-r.a; therefore, it activates Tbx6-r.b only in cells expressing Zic-r.a abundantly. Meanwhile, we found that Zic-r.a expressed zygotically in late embryos activates neural genes through canonical sites. Because different zinc-finger domains of Zic-r.a are important for driving reporters with canonical and non-canonical sites, it is likely that the non-canonical motif is not a divergent version of the canonical motif. In other words, our data indicate that the non-canonical motif represents a motif distinct from the canonical motif. Thus, Zic-r.a recognizes two distinct motifs to activate two sets of genes at two timepoints in development.

収録刊行物

• Development

  Development 148 (11), 2021-06

  The Company of Biologists

キーワード

• Ascidians
• Transcription factor
• Non-canonical-motif sites
• Zic
• Tbx6

詳細情報

• CRID: 1050570000404709632
• NII論文ID: 120007089266
• DOI: 10.1242/dev.199538
• ISSN: 14779129
• ISSN: 09501991
• HANDLE: 2433/263897
• Web Site: http://journals.biologists.com/dev/article-pdf/doi/10.1242/dev.199538/2025893/dev199538.pdf
• Web Site: http://journals.biologists.com/dev/article-pdf/doi/10.1242/dev.199538/2078739/dev199538.pdf
• 本文言語コード: ja
• 資料種別: journal article
• データソース種別:
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