Genome editing in plants using CRISPR type I-D nuclease

• Osakabe, Keishi

• Wada, Naoki

• Miyaji, Tomoko

• Murakami, Emi

• Marui, Kazuya

• Ueta, Risa

• Hashimoto, Ryosuke

• Abe-Hara, Chihiro

• Kong, Bihe

• Yano, Kentaro

• Osakabe, Yuriko

Abstract

Genome editing in plants has advanced greatly by applying the clustered regularly interspaced short palindromic repeats (CRISPRs)-Cas system, especially CRISPR-Cas9. However, CRISPR type I—the most abundant CRISPR system in bacteria—has not been exploited for plant genome modification. In type I CRISPR-Cas systems, e.g., type I-E, Cas3 nucleases degrade the target DNA in mammals. Here, we present a type I-D (TiD) CRISPR-Cas genome editing system in plants. TiD lacks the Cas3 nuclease domain; instead, Cas10d is the functional nuclease in vivo. TiD was active in targeted mutagenesis of tomato genomic DNA. The mutations generated by TiD differed from those of CRISPR/Cas9; both bi-directional long-range deletions and short indels mutations were detected in tomato cells. Furthermore, TiD can be used to efficiently generate bi-allelic mutant plants in the first generation. These findings indicate that TiD is a unique CRISPR system that can be used for genome engineering in plants.

Journal

• Communications Biology

  Communications Biology 3 648-, 2020-11-06

  Springer Nature

Keywords

• General Agricultural and Biological Sciences
• General Biochemistry, Genetics and Molecular Biology
• Medicine (miscellaneous)

Details

• CRID: 1050851475390598144
• NII Article ID: 120007089853
• ISSN: 23993642
• DOI: 10.1038/s42003-020-01366-6
• Web Site: http://repo.lib.tokushima-u.ac.jp/115886
• Web Site: https://www.nature.com/articles/s42003-020-01366-6.pdf
• Web Site: https://www.nature.com/articles/s42003-020-01366-6
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • Crossref
  • CiNii Articles
  • KAKEN