Blood Pressure-Lowering Peptides from Neo-Fermented Buckwheat Sprouts: A New Approach to Estimating ACE-Inhibitory Activity

• Koyama, Masahiro

• Hattori, Seiji

• Amano, Yoshihiko

• Watanabe, Masanori

• Nakamura, Kozo

• Luis Eduardo M. Quintas

  editor

抄録

Neo-fermented buckwheat sprouts (neo-FBS) contain angiotensin-converting enzyme (ACE) inhibitors and vasodilators with blood pressure-lowering (BPL) properties in spontaneously hypertensive rats (SHRs). In this study, we investigated antihypertensive mechanisms of six BPL peptides isolated from neo-FBS (FBPs) by a vasorelaxation assay and conventional in vitro, in vivo, and a new ex vivo ACE inhibitory assays. Some FBPs demonstrated moderate endothelium-dependent vasorelaxation in SHR thoracic aorta and all FBPs mildly inhibited ACE in vitro. Orally administered FBPs strongly inhibited ACE in SHR tissues. To investigate detailed ACE-inhibitory mechanism of FBPs in living body tissues, we performed the ex vivo assay by using endothelium-denuded thoracic aorta rings isolated from SHRs, which demonstrated that FBPs at low concentration effectively inhibited ACE in thoracic aorta tissue and suppressed angiotensin II-mediated vasoconstriction directly associated with BPL. These results indicate that the main BPL mechanism of FBP was ACE inhibition in living body tissues, suggesting that high FBP's bioavailability including absorption, tissue affinity, and tissue accumulation was responsible for the superior ACE inhibition in vivo. We propose that our ex vivo assay is an efficient and reliable method for evaluating ACE-inhibitory mechanism responsible for BPL activity in vivo.

Article

PLOS ONE. 9(9):e105802 (2014)

収録刊行物

• PLOS ONE

  PLOS ONE 9 (9), e105802-, 2014-09-15

  PUBLIC LIBRARY SCIENCE

キーワード

• Multidisciplinary

詳細情報

• CRID: 1050845763850908160
• NII論文ID: 120007106236
• DOI: 10.1371/journal.pone.0105802
• ISSN: 19326203
• HANDLE: 10091/18105
• Web Site: https://soar-ir.repo.nii.ac.jp/records/10232
• Web Site: http://dx.plos.org/10.1371/journal.pone.0105802
• 本文言語コード: en
• 資料種別: journal article
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