Effects of introduction of sorghum RbcS with rice RbcS knockdown by RNAi on photosynthetic activity and dry weight in rice

• Sakoda, Kazuma

• Yamamoto, Akito

• Ishikawa, Chie

• Taniguchi, Yojiro

• Matsumura, Hiroyoshi

• Fukayama, Hiroshi

Abstract

Rubisco is the key enzyme responsible for photosynthetic CO2 fixation. Previously, we succeeded to increase the catalytic rate (k (cat)) of Rubisco by the overexpression of sorghum C-4-type Rubisco small subunit (RbcS) in rice. These Rubisco are present as a chimera of sorghum RbcS and rice RbcS. In this study, we introduced an RNAi construct for rice RbcS into a sorghum RbcS overexpression line (SS10). Obtained double-transgenic lines iSS4 and iSS5 dominantly expressed sorghum RbcS and accumulated 35% and 20% of Rubisco content of non-transgenic rice (WT), respectively. Rubisco in these double-transgenic lines showed higher k (cat) and K (m) for CO2 than WT and SS10. Moreover, iSS4 showed higher photosynthetic nitrogen use efficiency under high CO2 conditions. These results demonstrate the usefulness of C-4-type RbcS to improve photosynthetic performance under elevated CO2 conditions.

Journal

• Plant Production Science

  Plant Production Science 24 (3), 346-353, 2021-07-03

  Taylor & Francis

Keywords

• C4 plant
• elevated CO2
• photosynthesis
• rice
• Rubisco
• RNAi

Details

• CRID: 1050856995321763328
• NII Article ID: 120007125729
• NII Book ID: AA11162156
• DOI: 10.1080/1343943x.2020.1847669
• ISSN: 13491008
• ISSN: 1343943X
• HANDLE: 20.500.14094/90008478
• Web Site: https://www.tandfonline.com/doi/pdf/10.1080/1343943X.2020.1847669
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • Crossref
  • CiNii Articles
  • KAKEN