Autophagy controls centrosome number by degrading Cep63

• Watanabe, Yuichiro

• Honda, Shinya

• Konishi, Akimitsu

• Arakawa, Satoko

• Murohashi, Michiko

• Yamaguchi, Hirofumi

• Torii, Satoru

• Tanabe, Minoru

• Tanaka, Shinji

• WARABI, Eiji

• Shimizu, Shigeomi

Abstract

Centrosome number is associated with the chromosome segregation and genomic stability. The ubiquitin–proteasome system is considered to be the main regulator of centrosome number. However, here we show that autophagy also regulates the number of centrosomes. Autophagy-deficient cells carry extra centrosomes. The autophagic regulation of centrosome number is dependent on a centrosomal protein of 63 (Cep63) given that cells lacking autophagy contain multiple Cep63 dots that are engulfed and digested by autophagy in wild-type cells, and that the upregulation of Cep63 increases centrosome number. Cep63 is recruited to autophagosomes via interaction with p62, a molecule crucial for selective autophagy. In vivo , hematopoietic cells from autophagy-deficient and p62 −/− mice also contained multiple centrosomes. These results indicate that autophagy controls centrosome number by degrading Cep63.

Journal

• Nature Communications

  Nature Communications 7 13508-, 2016-11

  Nature Research

Keywords

• General Physics and Astronomy
• General Biochemistry, Genetics and Molecular Biology
• General Chemistry
• Multidisciplinary

Details

• CRID: 1050001202619759488
• NII Article ID: 120007129257
• NII Book ID: AA12645905
• DOI: 10.1038/ncomms13508
• ISSN: 20411723
• HANDLE: 2241/00144549
• Web Site: https://tsukuba.repo.nii.ac.jp/records/39889
• Web Site: https://www.nature.com/articles/ncomms13508.pdf
• Web Site: https://www.nature.com/articles/ncomms13508
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • Crossref
  • CiNii Articles
  • KAKEN