Ultra-multiplex CARS spectroscopic imaging with 1-millisecond pixel dwell time

• 加納, 英明

• 野口, 雅之

• Maruyama, Takumi

• Kano, Junko

• Oka, Yuki

• Kaneta, Daiki

• Guerenne, Tiffany

• Leproux, Philippe

• Couderc, Vincent

抄録

We performed ultra-multiplex coherent anti-Stokes Raman scattering (CARS) spectroscopic imaging by using a CCD camera with a fast readout time (<1 ms). The ultra-multiplex CARS signal of a polystyrene bead was detected in the range 600–3600 cm-1 with resolution <10 cm-1. The pixel dwell time was approximately 1 ms, which was limited by the readout time of the CCD camera rather than the exposure time. CARS images of 161 × 161 pixels were obtained of the polymer beads with a total data-acquisition time of approximately 28 s even with the use of a cost-effective microchip laser source. Label-free and ultra-multiplex (18 colors) imaging of living cells was also performed with an effective exposure time of 1.8 ms based on the molecular fingerprint as well as the C-H and O-H stretching vibrational modes using a master oscillator fiber amplifier laser source.

収録刊行物

• OSA Continuum

  OSA Continuum 2 (5), 1693-, 2019-05

  Optical Society of America

キーワード

• Electrical and Electronic Engineering
• Atomic and Molecular Physics, and Optics
• Electronic, Optical and Magnetic Materials

詳細情報

• CRID: 1050282814175604352
• NII論文ID: 120007133554
• ISSN: 25787519
• DOI: 10.1364/osac.2.001693
• HANDLE: 2241/00157334
• Web Site: https://tsukuba.repo.nii.ac.jp/records/51904
• Web Site: https://www.osapublishing.org/viewmedia.cfm?URI=osac-2-5-1693&seq=0
• 本文言語コード: en
• 資料種別: journal article
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