Ca2+-permeable mechanosensitive channels MCA1 and MCA2 mediate cold-induced cytosolic Ca2+ increase and cold tolerance in Arabidopsis

• Mori, Kendo

• Renhu, Na

• Naito, Maho

• Nakamura, Aki

• Shiba, Hayato

• Yamamoto, Tsuyoshi

• SUZAKI, Takuya

• Iida, Hidetoshi

• MIURA, Kenji

Abstract

Cold shock triggers an immediate rise in the cytosolic free calcium concentration ([Ca 2+ ] cyt ) in Arabidopsis thaliana and this cold-induced elevation of [Ca 2+ ] cyt is inhibited by lanthanum or EGTA. It is suggested that intracellular calcium mainly contributes to the cold-induced [Ca 2+ ] cyt response by entering into the cytosol. Two calcium-permeable mechanosensitive channels, MCA1 and MCA2 ( mid1 -complementing activity), have been identified in Arabidopsis. Here, we demonstrate that MCA1 and MCA2 are involved in a cold-induced increase in [Ca 2+ ] cyt . The cold-induced [Ca 2+ ] cyt increase in mca1 and mca2 mutants was markedly lower than that in wild types. The mca1 mca2 double mutant exhibited chilling and freezing sensitivity, compared to wild-type plants. Expression of At5g61820 , At3g51660 , and At4g15490 , which are not regulated by the CBF/DREB1s transcription factor, was down-regulated in mca1 mca2 . These results suggest that MCA1 and MCA2 are involved in the cold-induced elevation of [Ca 2+ ] cyt , cold tolerance, and CBF/DREB1 -independent cold signaling.

Journal

• Scientific Reports

  Scientific Reports 8 550-, 2018-11

  Nature Publishing Group

Keywords

• Multidisciplinary

Details

• CRID: 1050282677537420416
• NII Article ID: 120007134505
• DOI: 10.1038/s41598-017-17483-y
• ISSN: 20452322
• HANDLE: 2241/00150981
• Web Site: https://tsukuba.repo.nii.ac.jp/records/45699
• Web Site: https://www.nature.com/articles/s41598-017-17483-y.pdf
• Web Site: https://www.nature.com/articles/s41598-017-17483-y
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • Crossref
  • CiNii Articles
  • KAKEN