Cysteinyl-tRNA synthetase governs cysteine polysulfidation and mitochondrial bioenergetics

DOI 機関リポジトリ HANDLE PDF Web Site 被引用文献98件 参考文献50件 オープンアクセス

この論文をさがす

抄録

<jats:title>Abstract</jats:title><jats:p>Cysteine hydropersulfide (CysSSH) occurs in abundant quantities in various organisms, yet little is known about its biosynthesis and physiological functions. Extensive persulfide formation is apparent in cysteine-containing proteins in <jats:italic>Escherichia coli</jats:italic> and mammalian cells and is believed to result from post-translational processes involving hydrogen sulfide-related chemistry. Here we demonstrate effective CysSSH synthesis from the substrate <jats:sc>l</jats:sc>-cysteine, a reaction catalyzed by prokaryotic and mammalian cysteinyl-tRNA synthetases (CARSs). Targeted disruption of the genes encoding mitochondrial CARSs in mice and human cells shows that CARSs have a crucial role in endogenous CysSSH production and suggests that these enzymes serve as the principal cysteine persulfide synthases in vivo. CARSs also catalyze co-translational cysteine polysulfidation and are involved in the regulation of mitochondrial biogenesis and bioenergetics. Investigating CARS-dependent persulfide production may thus clarify aberrant redox signaling in physiological and pathophysiological conditions, and suggest therapeutic targets based on oxidative stress and mitochondrial dysfunction.</jats:p>

収録刊行物

被引用文献 (98)*注記

もっと見る

参考文献 (50)*注記

もっと見る

関連プロジェクト

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ