Effects of whole blood storage in a polyolefin blood bag on platelets for acute normovolemic hemodilution

• 村田, 隆

• Kusudo, Eriko

• 川本, 修司

• 福田, 和彦

抄録

Acute normovolemic hemodilution (ANH) is a potential transfusion method for platelets, as well as for red blood cells. However, previous studies have shown that whole blood storage in ANH decreases platelet aggregability by 14.7–76.3% and that this decrease is not recovered by reinfusion. We investigated whether a new whole blood storage method for 6 h using a polyolefin bag, based on the platelet concentrates storage method, would maintain platelet function better than the conventional method using a polyvinyl chloride bag. We demonstrated that storage of whole blood in a polyolefin bag maintained ADP-induced aggregation rates at more than twofold higher than those in a polyvinyl chloride bag, and also significantly suppressed P-selectin expression, a platelet activation marker (ADP-induced aggregation rates: 24.6 ± 5.1% vs. 51.7 ± 11.5%, p = 0.002; P-selectin expression; 50.3 ± 8.4MFI vs. 31.6 ± 9.3MFI, p = 0.018). These results could be attributed to the high gas permeability of polyolefin, which lowered PCO2 and maintained a high pH with or without agitation. There were no significant changes in platelet count and red blood cell parameters due to the storage methods. Our results suggest that ANH using polyolefin bags is advantageous in improving hemostatic function compared to the conventional method.

収録刊行物

• Scientific Reports

  Scientific Reports 11 2021

  Springer Nature

キーワード

• Multidisciplinary

詳細情報

• CRID: 1050289321203332992
• NII論文ID: 120007142018
• DOI: 10.1038/s41598-021-91725-y
• HANDLE: 2433/264438
• ISSN: 20452322
• Web Site: https://www.nature.com/articles/s41598-021-91725-y.pdf
• Web Site: https://www.nature.com/articles/s41598-021-91725-y
• 本文言語コード: en
• 資料種別: journal article
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