In Vivo Suppression of Osteoclastic and Osteoblastic Activities of Goldfish Scales in Water Containing Cadmium

DOI HANDLE Open Access
  • SUZUKI Nobuo
    Noto Marine Laboratory, Institute of Nature and Environmental Technology, Kanazawa University
  • KAWAGO Umi
    Noto Marine Laboratory, Institute of Nature and Environmental Technology, Kanazawa University
  • HONDA Masato
    Botanical Garden, Institute of Nature and Environmental Technology, Kanazawa University
  • SRIVASTAV Ajai K.
    Department of Zoology, DDU Gorakhpur University
  • AMORNSAKUN Thumronk
    Fisheries Technology Program, Department of Technology and Industries, Faculty of Sciences and Technology
  • MATSUMOTO Kyoko
    Institute of Noto Satoumi Education and Studies
  • HIRAYAMA Jun
    Department of Clinical Engineering, Faculty of Health Sciences, Komatsu University
  • MATSUBARA Hajime
    Noto Center for Fisheries Science and Technology, Kanazawa University
  • SHIMIZU Nobuaki
    Noto Marine Laboratory, Institute of Nature and Environmental Technology, Kanazawa University Faculty of Pharmaceutical Sciences, Nagasaki International University
  • SEKIGUCHI Toshio
    Noto Marine Laboratory, Institute of Nature and Environmental Technology, Kanazawa University
  • SASAYAMA Yuichi
    Noto Marine Laboratory, Institute of Nature and Environmental Technology, Kanazawa University
  • TABUCHI Yoshiaki
    Division of Molecular Genetics Research, Life Science Research Center, University of Toyama
  • HATTORI Atsuhiko
    Department of Biology, College of Liberal Arts and Sciences, Tokyo Medical and Dental University
  • SHIMASAKI Yohei
    Laboratory of Marine Environmental Science, Division of Animal & Marine Bioresource Science, Department of Bioresource Sciences, Faculty of Agriculture, Kyushu University
  • OSHIMA Yuji
    Laboratory of Marine Environmental Science, Division of Animal & Marine Bioresource Science, Department of Bioresource Sciences, Faculty of Agriculture, Kyushu University

Search this article

Abstract

The fish scales are well known as a major source of internal calcium storage. Therefore, we developed an original assay system using goldfish scales in which both osteoclasts (bone resorption cells) and osteoblasts (bone formation cells) coexist. In our bioassay system, we utilized the activities of tartrate–resistant acid phosphatase (TRAP) and alkaline phosphatase (ALP) as respective indicators of each activity in osteoclasts and osteoblasts. Using this bioassay system, the influence of cadmium chloride (CdCl_2) on osteoclastic and osteoblastic activities in the scales of goldfish was examined in an in vivo experiment. Goldfish were kept in tap water containing CdCl_2 (10^–7 M) for 2 days. TRAP activity in the scales of goldfish decreased with 2 days of exposure to CdCl_2. This inhibitory effect for osteoclasts continued even at 4 days of exposure to CdCl2. In the case of osteoblasts, CdCl2 inhibited ALP activity in the goldfish scales at 4 days after exposure, although ALP activity in the goldfish scales had not changed after 2 days of exposure to CdCl_2. In in vitro cultured goldfish scales, we previously reported that ALP activity decreased after exposures of 64 and 96 hrs, although their activities did not change after 6, 18, and 36 hrs. These results were supported by our in vivo experiment. This is the first report to indicate that both osteoclasts and osteoblasts in fish were suppressed by cadmium (Cd) treatments in vivo. Considering both in vivo and in vitro experiments, we concluded that Cd inhibited both osteoclastic and osteoblastic activities in goldfish. This suggests that Cd leads to a disturbed calcium metabolism and then induces bone anomalies.

Journal

Related Projects

See more

Keywords

Details 詳細情報について

Report a problem

Back to top