Optimized sonoreactor for accelerative amyloid-fibril assays through enhancement of primary nucleation and fragmentation

• Nakajima, Kichitaro

• Noi, Kentaro

• Yamaguchi, Keiichi

• So, Masatomo

• Ikenaka, Kensuke

• Mochizuki, Hideki

• Ogi, Hirotsugu

• Goto, Yuji

Abstract

Nakajima K., Noi K., Yamaguchi K., et al. Optimized sonoreactor for accelerative amyloid-fibril assays through enhancement of primary nucleation and fragmentation. Ultrasonics Sonochemistry, 73, 105508, 2021. https://doi.org/10.1016/j.ultsonch.2021.105508.

Ultrasonication to supersaturated protein solutions forcibly forms amyloid fibrils, thereby allowing the early-stage diagnosis for amyloidoses. Previously, we constructed a high-throughput sonoreactor to investigate features of the amyloid-fibril nucleation. Although the instrument substantiated the ultrasonication efficacy, several challenges remain; the key is the precise control of the acoustic field in the reactor, which directly affects the fibril-formation reaction. In the present study, we develop the optimized sonoreactor for the amyloid-fibril assay, which improves the reproducibility and controllability of the fibril formation. Using β2-microglobulin, we experimentally demonstrate that achieving identical acoustic conditions by controlling oscillation amplitude and frequency of each transducer results in identical fibril-formation behavior across 36 solutions. Moreover, we succeed in detecting the 100-fM seeds using the developed sonoreactor at an accelerated rate. Finally, we reveal that the acceleration of the fibril-formation reaction with the seeds is achieved by enhancing the primary nucleation and the fibril fragmentation through the analysis of the fibril-formation kinetics. These results demonstrate the efficacy of the developed sonoreactor for the diagnosis of amyloidoses owing to the accelerative seed detection and the possibility for further early-stage diagnosis even without seeds through the accelerated primary nucleation.

Journal

• Ultrasonics Sonochemistry

  Ultrasonics Sonochemistry 73 105508-, 2021-03-03

  Elsevier B.V.

Keywords

• Amyloid fibril
• Fragmentation
• Nucleation
• Sonocrystallization
• Sonoreactor
• Supersaturation

Details

• CRID: 1050581168900555520
• NII Article ID: 120007148338
• NII Book ID: AA11024447
• DOI: 10.1016/j.ultsonch.2021.105508
• ISSN: 18732828
• ISSN: 13504177
• HANDLE: 11094/84201
• Web Site: https://api.elsevier.com/content/article/PII:S135041772100050X?httpAccept=text/xml
• Web Site: https://api.elsevier.com/content/article/PII:S135041772100050X?httpAccept=text/plain
• Text Lang: en
• Article Type: journal article
• Data Source:
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