Time-resolved observation of evolution of amyloid-beta oligomer with temporary salt crystals

• Nakajima, Kichitaro

• Yamazaki, Tomoya

• Kimura, Yuki

• So, Masatomo

• Goto, Yuji

• Ogi, Hirotsugu

書誌事項

タイトル別名

• Time-Resolved Observation of Evolution of Amyloid-β Oligomer with Temporary Salt Crystals

抄録

Nakajima K., Yamazaki T., Kimura Y., et al. "Time-Resolved Observation of Evolution of Amyloid-β Oligomer with Temporary Salt Crystals", Journal of Physical Chemistry Letters, 11(15), 6176-6184, July 20, 2020. Copyright © 2020 American Chemical Society. https://doi.org/10.1021/acs.jpclett.0c01487.

The aggregation behavior of amyloid-β (Aβ) peptides remains unclarified despite the fact that it is closely related to the pathogenic mechanism of Alzheimer's disease. Aβ peptides form diverse oligomers with various diameters before nucleation, making clarification of the mechanism involved a complex problem with conventional macroscopic analysis methods. Time-resolved single-molecule level analysis in bulk solution is thus required to fully understand their early stage aggregation behavior. Here, we perform time-resolved observation of the aggregation dynamics of Aβ oligomers in bulk solution using liquid-state transmission electron microscopy. Our observations reveal previously unknown behaviors. The most important discovery is that a salt crystal can precipitate even with a concentration much lower than its solubility, and it then dissolves in a short time, during which the aggregation reaction of Aβ peptides is significantly accelerated. These findings will provide new insights in the evolution of the Aβ oligomer.

収録刊行物

• Journal of Physical Chemistry Letters

  Journal of Physical Chemistry Letters 11 (15), 6176-6184, 2020-07-20

  American Chemical Society

キーワード

• General Materials Science
• Physical and Theoretical Chemistry

詳細情報

• CRID: 1050581168900561536
• NII論文ID: 120007148352
• NII書誌ID: AA1260018X
• DOI: 10.1021/acs.jpclett.0c01487
• ISSN: 19487185
• HANDLE: 11094/84186
• Web Site: https://pubs.acs.org/doi/pdf/10.1021/acs.jpclett.0c01487
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • Crossref
  • CiNii Articles
  • KAKEN