Alteration of transbilayer phospholipid compositions is involved in cell adhesion, cell spreading, and focal adhesion formation

• 宮野, 吏永

• 松本, 尚

• 高津, 宏之

• 中山, 和久

• 申, 惠媛

抄録

We previously showed that P4-ATPases, ATP10A/ATP8B1, and ATP11A/ATP11C have flippase activities toward phosphatidylcholine (PC), and aminophospholipids [phosphatidylserine (PS) and phosphatidylethanolamine], respectively. Here, we investigate the effect of PC-specific flippases versus aminophospholipid-specific flippases in cell spreading on the extracellular matrix. Expression of PC-flippases, but not PS-flippases, delayed cell adhesion, cell spreading and inhibited formation of focal adhesions. In addition, overexpression of a PS-binding probe that sequesters PS in the cytoplasmic leaflet delayed cell spreading and inhibited formation of focal adhesions. These results suggest that elevation of PC at the cytoplasmic leaflet of the plasma membrane by expression of PC-flippases may reduce the local concentration of PS or phosphoinositides, required for efficient cell adhesion, focal adhesion formation, and cell spreading.

収録刊行物

• FEBS Letters

  FEBS Letters 590 (14), 2138-2145, 2016-07

  Wiley

キーワード

• ATPase
• cell spreading
• flippase
• focal adhesion
• lipid bilayer
• phospholipid

詳細情報

• CRID: 1050008212372796160
• NII論文ID: 120007159976
• ISSN: 18733468
• ISSN: 00145793
• HANDLE: 2433/265375
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles