Structural basis for high selectivity of a rice silicon channel Lsi1

• Saitoh, Yasunori

• Mitani-Ueno, Namiki

• Saito, Keisuke

• Matsuki, Kengo

• Huang, Sheng

• Yang, Lingli

• Yamaji, Naoki

• Ishikita, Hiroshi

• Shen, Jian-Ren

• Ma, Jian Feng

• Suga, Michihiro

抄録

Silicon (Si), the most abundant mineral element in the earth’s crust, is taken up by plant roots in the form of silicic acid through Low silicon rice 1 (Lsi1). Lsi1 belongs to the Nodulin 26-like intrinsic protein subfamily in aquaporin and shows high selectivity for silicic acid. To uncover the structural basis for this high selectivity, here we show the crystal structure of the rice Lsi1 at a resolution of 1.8 Å. The structure reveals transmembrane helical orientations different from other aquaporins, characterized by a unique, widely opened, and hydrophilic selectivity filter (SF) composed of five residues. Our structural, functional, and theoretical investigations provide a solid structural basis for the Si uptake mechanism in plants, which will contribute to secure and sustainable rice production by manipulating Lsi1 selectivity for different metalloids.

収録刊行物

• Nature Communications

  Nature Communications 12 (1), 6236-, 2021-10-29

  Nature Portfolio

キーワード

• General Physics and Astronomy
• General Biochemistry, Genetics and Molecular Biology
• General Chemistry
• Multidisciplinary

詳細情報

• CRID: 1050571638774929280
• NII論文ID: 120007170514
• ISSN: 20411723
• DOI: 10.1038/s41467-021-26535-x
• Web Site: https://ousar.lib.okayama-u.ac.jp/62861
• Web Site: https://www.nature.com/articles/s41467-021-26535-x.pdf
• Web Site: https://www.nature.com/articles/s41467-021-26535-x
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • Crossref
  • CiNii Articles
  • KAKEN