Elevated Myeloperoxidase-DNA Complex Levels in Sera of Patients with IgA Vasculitis

• Takeuchi, Sora

• Kawakami, Tamihiro

• Okano, Tatsuro

• Shida, Haruki

• Nakazawa, Daigo

• Tomaru, Utano

• Ishizu, Akihiro

• Kadono, Takafumi

Abstract

Introduction: IgA vasculitis is a systemic disease that results from the entrapment of circulating IgA-containing immune complexes in small-vessel walls in the skin, kidneys, and gastrointestinal tract. An excessive formation of neutrophil extracellular traps (NETs) is involved in the pathogenesis of vasculitis, especially in antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis. This study aimed to clarify whether NETs are implicated in IgA vasculitis. Methods: Twenty-two patients with IgA vasculitis and 4 healthy volunteers were enrolled in this study. Serum levels of myeloperoxidase (MPO)-DNA complex, a fragment derived from NETs, were determined by enzyme-linked immunosorbent assay (ELISA), and the association between MPO-DNA complex levels and clinical parameters was examined. The presence of the ANCA was also assessed by ELISA specific for MPO and proteinase 3 (PR3) and indirect immunofluorescence (IIF), followed by assessing the differences in clinical parameters with and without the ANCA. Results: Serum MPO-DNA complex levels were significantly higher in patients with IgA vasculitis than those in healthy controls. A significant positive correlation between the serum MPO-DNA complex and IgA levels was noted. Interestingly, 63.6% of IgA vasculitis patients were ANCA-positive in IIF with an atypical pattern, whereas neither MPO-ANCA nor PR3-ANCA was detected by ELISA. These findings indicated that some IgA vasculitis patients possessed the so called minor ANCA. Serum IgA and MPO-DNA complex levels and the frequency of hematuria in the minor ANCA-positive group were significantly higher than in the minor ANCA-negative group. Conclusion: The collective findings suggested that NETs are certainly involved in the pathogenesis of IgA vasculitis.

Journal

• Pathobiology

  Pathobiology 89 (1), 23-28, 2021-11-23

  Karger

Keywords

• IgA vasculitis
• Neutrophil extracellular traps
• Antineutrophil cytoplasmic antibody
• Renal complications

Details

• CRID: 1050571782547628416
• NII Article ID: 120007173221
• DOI: 10.1159/000519869
• ISSN: 14230291
• ISSN: 10152008
• HANDLE: 2115/83384
• Web Site: https://www.karger.com/Article/Pdf/519869
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • Crossref
  • CiNii Articles
  • KAKEN