Size-Controlled Production of Gold Bionanoparticles Using the Extremely Acidophilic Fe(III)-Reducing Bacterium, Acidocella aromatica

• Intan Nurul Rizki

  九州大学大学院工学府地球資源システム工学専攻

• 沖部, 奈緒子

  九州大学大学院工学研究院地球資源システム工学部門

抄録

Recycling of gold-bearing “urban mine” resources, such as waste printed circuit boards (PCBs), is attracting an increasing interest. Some of the gold leaching techniques utilize acidic lixiviants and in order to eventually target such acidic leachates, the utility of the acidophilic Fe(III)-reducing heterotrophic bacterium, Acidocella (Ac.) aromatica PFBC was evaluated for production of Au(0) bionanoparticles (bio-AuNPs). Au(III) ions (as AuCl_4-, initially 10 mg/L), were readily adsorbed onto the slightly-positively charged Ac. aromatica cell surface and transported into cytoplasm to successfully form intracellular bio-AuNPs in a simple one-step microbiological reaction. Generally, increasing the initial concentration of formate as e-donor corresponded to faster Au(III) bioreduction and a greater number of Au(0) nucleation sites with less crystal growth within 40–60 h: i.e., use of 1, 5, 10, or 20 mM formate led to production of bio-AuNPs of 48, 24, 13, or 12 nm in mean particle size with 2.3, 17, 62, and 97 particles/cell, respectively. Addition of Cu^2+ as an enzymatic inhibitor significantly decreased the number of Au(0) nucleation sites but enhanced crystal growth of individual particles. As a result, the manipulation of the e-donor concentration combined with an enzyme inhibitor enabled the 3-grade size-control of bio-AuNPs (nearly within a normal distribution) at 48, 26 or 13 nm by use of 1 mM formate, 20 mM formate (+Cu^2+) or 10 mM formate, respectively, from highly acidic, dilute Au(III) solutions.

収録刊行物

• Minerals

  Minerals 8 (3), 81-, 2018-02-26

  MDPI

キーワード

• gold nanoparticles
• Acidocella aromatica PFBC
• Fe(III)-reducing bacterium
• acidophilic microorganism
• size-control

詳細情報

• CRID: 1050580007680323712
• NII論文ID: 120007174034
• ISSN: 2075163X
• HANDLE: 2324/4737405
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles