Nobiletin Inhibits Inflammatory Reaction in Interleukin-1β-Stimulated Human Periodontal Ligament Cells

• Hosokawa, Yoshitaka

• Hosokawa, Ikuko

• Ozaki, Kazumi

• Matsuo, Takashi

抄録

The immune response in periodontal lesions is involved in the progression of periodontal disease. Therefore, it is important to find a bioactive substance that has anti-inflammatory effects in periodontal lesions. This study aimed to examine if nobiletin, which is found in the peel of citrus fruits, could inhibit inflammatory responses in interleukin (IL)-1β-stimulated human periodontal ligament cells (HPDLCs). The release of cytokines (IL-6, IL-8, CXCL10, CCL20, and CCL2) and matrix metalloproteinases (MMP-1 and MMP-3) was assessed by ELISA. The expression of cell adhesion molecules (ICAM-1and VCAM-1) and the activation of signal transduction pathways (nuclear factor (NF)-κB, mitogen-activated protein kinases (MAPKs) and protein kinase B (Akt)) in HPDLCs were detected by Western blot analysis. Our experiments revealed that nobiletin decreased the expression of inflammatory cytokines, cell adhesion molecules, and MMPs in IL-1β-stimulated HPDLCs. Moreover, we revealed that nobiletin treatment could suppress the activation of the NF-κB, MAPKs, and Akt pathways. These findings indicate that nobiletin could inhibit inflammatory reactions in IL-1β-stimulated HPDLCs by inhibiting multiple signal transduction pathways, including NF-κB, MAPKs, and Akt.

収録刊行物

• Pharmaceutics

  Pharmaceutics 13 (5), 667-, 2021-05-07

  MDPI

キーワード

• nobiletin
• anti-inflammatory effect
• periodontal ligament cells

詳細情報

• CRID: 1050853986994217728
• NII論文ID: 120007188425
• ISSN: 19994923
• DOI: 10.3390/pharmaceutics13050667
• Web Site: http://repo.lib.tokushima-u.ac.jp/116619
• Web Site: https://www.mdpi.com/1999-4923/13/5/667/pdf
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
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