Effects of Temporary Blood Administration on Dysoxia and Survival in a Rat Uncontrolled Hemorrhagic Shock Model

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Abstract

Objective: To test whether temporary blood administration improves dysoxia and thereby prolong survival in an ongoing uncontrolled hemorrhagic shock model. Methods: Light anesthesia was induced with sevoflurane in 18 rats, and spontaneous breathing was maintained. Uncontrolled hemorrhagic shock (UHS) was induced by withdrawal of blood at 2.5 mL / 100 g over a 15-min period, followed by 75 % tail amputation. At 10 min after tail cutting, rats were randomized into three groups (n = 6 each) and received the following resuscitation regimen for 20 min: Group 1 (9 mL shed blood) vs. Group 2 (a mixture of 4.5 mL NS solution and 4.5 mL shed blood) vs. Group 3 (9 mL NS solution). The rats were then monitored for oxygen metabolisms and survival. Results: The regimen of Group 1 vs. Group 3 prompted a surge in blood pressure and improved oxygen metabolic indices. Four rats in Group 1, three rats in Group 2, and none in Group 3 survived up to 180 min (p = 0.052; Group 1 vs. Group 3). Additional blood loss from the tail stump did not differ significantly among the groups. Conclusions: In a model of UHS in rats, a temporary resuscitation regimen of whole blood administration compared with NS solution improved tissue dysoxia and possibly survival.

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