Continuous Fermentation of L-Lactic Acid by the Mold Rhizopus oryzae

• KOIDE Michiteru

  Department of Applied Chemistry, Oita University

• HIRATA Makoto

  Department of Applied Chemistry, Oita University

• GAO Min-tian

  Department of Applied Chemistry, Oita University

• TAKANASHI Hirokazu

  Department of Bioengineering, Kagoshima University

• HANO Tadashi

  Department of Applied Chemistry, Oita University

Bibliographic Information

Other Title

• 糸状菌Ｒｈｉｚｏｐｕｓ　ｏｒｙｚａｅによるＬ‐乳酸の連続発酵
• 糸状菌Rhizopus oryzaeによるL-乳酸の連続発酵
• シジョウキン Rhizopus oryzae ニ ヨル L ニュウサン ノ レンゾク ハッコウ

Abstract

The continuous fermentation of L-lactic acid by Rhizopus oryzae AHU6537 was attempted with dilution rate of 0.02 h^-1 and feed glucose rate of 2.0 kg/(m³·h) in an airlift bioreactor. As a result, stable operation could be carried out over a long time without immobilizing cells. When the optimum amount of yeast extract in batch culture was added to the starting medium, the cells formed spherical pellets, and the fermentation rate was improved. Further, to investigate the influence of glucose and lactic acid concentration, when the dilution rate and glucose concentration in feed medium were controlled in continuous fermentation, it was found that R. oryzea was strongly inhibited by the lactic acid produced. When the effect of lactic acid inhibition was analyzed according to a Michaelis-Menten mechanism with uncompetitive inhibition and the fermentation rate at the steady state was simulated, the calculated lines agreed well with experimental values.

Journal

• KAGAKU KOGAKU RONBUNSHU

  KAGAKU KOGAKU RONBUNSHU 30 (4), 432-439, 2004

  The Society of Chemical Engineers, Japan

Keywords

• L-Lactic Acid
• Poly Lactic Acid
• <i>Rhizopus oryzae</i>
• Continuous Fermentation
• Product Inhibition

Details

• CRID: 1390282679484659712
• NII Article ID: 130000018724
• NII Book ID: AN00037234
• DOI: 10.1252/kakoronbunshu.30.432
• ISSN: 13499203
• ISSN: 0386216X
• NDL BIB ID: 7047298
• Web Site: http://id.ndl.go.jp/bib/7047298
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7047298
• Web Site: http://www.jstage.jst.go.jp/article/kakoronbunshu/30/4/30_4_432/_pdf
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed