Activity of .BETA.-galactosidase solubilized in reverse micelles and selective back-extraction from micellar phase.
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- Shiomori Koichiro
- Department of Materials Science, Miyazaki University
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- Kawano Yoshinobu
- Department of Materials Science, Miyazaki University
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- Kuboi Ryoichi
- Department of Chemical Engineering, Osaka University
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- Komasawa nd Isao
- Department of Chemical Engineering, Osaka University
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Solubilization of β-galactosidase into AOT/isooctane reverse micelles was achieved by the injection method while holding its activity. The observed activity in reverse micelles was influenced by the water content Wo. Two peaks in the initial activity were observed, at Wo = 10 and 45. Enzyme stability increased with Wo value. The activity and the stability of β-galactosidase in reverse micelles are interpreted by the solubilization mechanism of proteins, considering micelle size distribution. Selective back-extraction of solubilized β-galactosidase from the micellar phase into a new aqueous phase was achieved by using 0.1 M KCl aqueous solution with high activity yield. The separation of β-galactosidase from E. coli cells was also successfully carried out by the present method where the solubilization of the crude extracts into reverse micelles by the injection method was followed by the next selective back-extraction from the reverse micelles. Enzyme was purified four-fold over the initial crude extract with high activity yield.
収録刊行物
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- JOURNAL OF CHEMICAL ENGINEERING OF JAPAN
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JOURNAL OF CHEMICAL ENGINEERING OF JAPAN 27 (3), 410-414, 1994
公益社団法人 化学工学会
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詳細情報 詳細情報について
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- CRID
- 1390282679541705856
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- NII論文ID
- 130000021337
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- NII書誌ID
- AA00709658
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- COI
- 1:CAS:528:DyaK2cXlsleltrc%3D
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- ISSN
- 18811299
- 00219592
- http://id.crossref.org/issn/00219592
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可