Induction of Egr-1 Is Associated with Anti-Metastatic and Anti-Invasive Ability of .BETA.-Lapachone in Human Hepatocarcinoma Cells

この論文にアクセスする

この論文をさがす

著者

    • Kim Sung Ok KIM Sung Ok
    • Department of Biomaterial Control (BK21 Program), Dongeui University Graduate School, Dongeui University College of Oriental Medicine
    • KIM Gi Young
    • Faculty of Applied Marine Science, Cheju National University
    • KIM Nam Deuk
    • Division of Pharmacy (BK21 Program), Pusan National University, Research Institute for Drug Development
    • CHOI Yung Hyun
    • Department of Biomaterial Control (BK21 Program), Dongeui University Graduate School, Dongeui University College of Oriental Medicine|Department of Biochemistry, Dongeui University College of Oriental Medicine

抄録

β-lapachone, a quinone compound obtained from the bark of the lapacho tree (<I>Tabebuia avellanedae</I>), was reported to have anti-inflammatory and anti-cancer activities. In this study, we investigated novel functions of β-lapachone in terms of anti-metastasis and anti-invasion abilities using human hepatocarcinoma cell lines, HepG2 and Hep3B. β-lapachone dose-dependently inhibited cell viability and migration of both HepG2 and Hep3B cells, as determined by methylthiazoletetrazolium (MTT) assay and wound healing assay. RT-PCR and Western blot data revealed that β-lapachone dramatically increased the levels of protein, as well as mRNA expression of early growth response gene-1 (Egr-1) and throbospondin-1 (TSP-1) at an early point in time, and then decreased in a time-dependent manner. In addition, down-regulation of Snail and up-regulation of E-cadherin expression were observed in β-lapachone-treated HepG2 and Hep3B cells, and this the associated with decreased invasive ability as measured by matrigel invasion assay. Taken together, our results strongly suggest that β-lapachone may be expected to inhibit the progression and metastasis of hepatoma cells, at least in part by inhibiting the invasive ability of the cells <I>via</I> up-regulation of the expression of the Egr-1, TSP-1, and E-cadherin.

収録刊行物

  • Bioscience, Biotechnology, and Biochemistry

    Bioscience, Biotechnology, and Biochemistry 71(9), 2169-2176, 2007

    公益社団法人 日本農芸化学会

各種コード

  • NII論文ID(NAID)
    130000022160
  • NII書誌ID(NCID)
    AA10824164
  • 本文言語コード
    ENG
  • ISSN
    0916-8451
  • NDL 記事登録ID
    8933673
  • NDL 雑誌分類
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-G223
  • データ提供元
    NDL  J-STAGE 
ページトップへ