Induction of Egr-1 is associated with anti-metastatic and anti-invasive ability of β-lapachone in human hepatocarcinoma cells

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  • KIM Sung Ok
    Department of Biomaterial Control (BK21 Program), Dongeui University Graduate School, Dongeui University College of Oriental Medicine
  • KWON Jae Im
    Department of Biochemistry, Dongeui University College of Oriental Medicine
  • JEONG Yong Kee
    College of Natural Resources and Life Science, Dong-A University
  • KIM Gi Young
    Faculty of Applied Marine Science, Cheju National University
  • KIM Nam Deuk
    Division of Pharmacy (BK21 Program), Pusan National University, Research Institute for Drug Development
  • CHOI Yung Hyun
    Department of Biomaterial Control (BK21 Program), Dongeui University Graduate School, Dongeui University College of Oriental Medicine Department of Biochemistry, Dongeui University College of Oriental Medicine

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タイトル別名
  • Induction of Egr-1 Is Associated with Anti-Metastatic and Anti-Invasive Ability of .BETA.-Lapachone in Human Hepatocarcinoma Cells
  • Induction of Egr 1 is associated with anti metastatic and anti invasive ability of v lapachone in human hepatocarcinoma cells

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β-lapachone, a quinone compound obtained from the bark of the lapacho tree (Tabebuia avellanedae), was reported to have anti-inflammatory and anti-cancer activities. In this study, we investigated novel functions of β-lapachone in terms of anti-metastasis and anti-invasion abilities using human hepatocarcinoma cell lines, HepG2 and Hep3B. β-lapachone dose-dependently inhibited cell viability and migration of both HepG2 and Hep3B cells, as determined by methylthiazoletetrazolium (MTT) assay and wound healing assay. RT-PCR and Western blot data revealed that β-lapachone dramatically increased the levels of protein, as well as mRNA expression of early growth response gene-1 (Egr-1) and throbospondin-1 (TSP-1) at an early point in time, and then decreased in a time-dependent manner. In addition, down-regulation of Snail and up-regulation of E-cadherin expression were observed in β-lapachone-treated HepG2 and Hep3B cells, and this the associated with decreased invasive ability as measured by matrigel invasion assay. Taken together, our results strongly suggest that β-lapachone may be expected to inhibit the progression and metastasis of hepatoma cells, at least in part by inhibiting the invasive ability of the cells via up-regulation of the expression of the Egr-1, TSP-1, and E-cadherin.

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