Purification and some properties of melanoidin-decolorizing enzymes, P-III and P-IV from mycelia of Coriolus versicolor Ps4a

• OHMOMO Sadahiro

  Institute of Applied Biochemistry, University of Tsukuba

• AOSHIMA Ikuko

  Department of food and Nutrition Science, Faculty of Home Economics, Japan Women's University

• TOZAWA Yukiko

  Institute of Applied Biochemistry, University of Tsukuba

• SAKURADA Noriko

  Institute of Applied Biochemistry, University of Tsukuba

• UEDA Kiyomoto

  Institute of Applied Biochemistry, University of Tsukuba

書誌事項

タイトル別名

• Research on the decolorization of melanoidin by microorganisms. Part II. Purification and some properties of melanoidin decolorizing enzymes, P-III and P-IV, from mycelia of Coriolus versicolor Ps4a.
• Purification and some properties of melanoidin decolorizing enzymes, P-III and P-I, from mycelia of Coriolus versicolor Ps4a

抄録

Melanoidin decolorizing enzymes (MDE) were extracted from mycelia of Coriolus versicolor Ps4a and purified by DEAE-Sephadex, DEAE-Sephacel and Sephadex G-200 column chromatographies. MDE of this strain consisted of a main fraction, P-fraction, and a minor fraction, E-fraction, and the P-fraction was composed of at least five enzymes. P-III and P-IV in the P-fraction were picked as typical enzymes of this strain, and their enzymatic properties were investigated. P-III had a molecular weight of 48, 400-50, 000, an optimum pH of 5.5 and an optimum temperature of 30-35°C. P-III required glucose and O₂ for the appearance of the activity, and was inhibited by p-CMB, N-BSI, Ag⁺ and o-phenanthroline.<br> On the other hand, P-IV had a molecular weight of 43, 800-45, 000, an optimum pH of 4.0-4.5 and an optimum temperature of 30-35°C. P-IV could decolorize melanoidin in the absence of glucose and O₂, and was inhibited weakly by Ag⁺, p-CME and N-BSI. P-IV is the enzyme that attacks the melanoidin directly in comparison with P-III which attacks melanoidin indirectly as in the sub-reaction of sugar oxidase.<br> Incidentally, a multiplicative effect between P-III and P-IV for decolorization was observed.

収録刊行物

• Agricultural and Biological Chemistry

  Agricultural and Biological Chemistry 49 (7), 2047-2053, 1985

  公益社団法人 日本農芸化学会

キーワード

• General Agricultural and Biological Sciences
• General Biochemistry, Genetics and Molecular Biology

詳細情報

• CRID: 1390001206465460608
• NII論文ID: 130000026183
• DOI: 10.1271/bbb1961.49.2047
• COI: 1:CAS:528:DyaL2MXlsFKhsLg%3D
• ISSN: 18811280
• ISSN: 00021369
• ISSN: http://id.crossref.org/issn/14316730
• ISSN: http://id.crossref.org/issn/00021369
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可