Enzymatic synthesis of trehalose from maltose.

• MURAO Sawao

  Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture

• NAGANO Hiroto

  Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture

• OGURA Sei

  Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture

• NISHINO Toyokazu

  Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture

抄録

Synthesis of trehalose from maltose by a coupled enzyme system with trehalose phosphorylase and maltose phosphorylase has been studied. Trehalose phosphorylase was partially purified from Euglena gracilis and maltose phosphorylase was obtained from Lactobacillus brevis. The optimum pH of the reaction was 6.5-7.0 and the reaction rate was faster in the rection mixture containing a low concentration of phosphate. The final ratio of conversion (the ratio of trehalose to maltose) in the pH range between 6.0 and 8.0 was about 60%.<br> Immobilized maltose and trehalose phosphorylase in κ-carageenan could be used without any appreciable loss of activity for batch reactions at least 10 times.

収録刊行物

• Agricultural and Biological Chemistry

  Agricultural and Biological Chemistry 49 (7), 2113-2118, 1985

  公益社団法人 日本農芸化学会

キーワード

• General Agricultural and Biological Sciences
• General Biochemistry, Genetics and Molecular Biology

詳細情報

• CRID: 1390001206464037248
• NII論文ID: 130000026192
• DOI: 10.1271/bbb1961.49.2113
• COI: 1:CAS:528:DyaL2MXlsFKmu7c%3D
• ISSN: 18811280
• ISSN: 00021369
• ISSN: http://id.crossref.org/issn/00021369
• 本文言語コード: en
• データソース種別:
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• 抄録ライセンスフラグ: 使用不可