One-step purification of guinea pig liver transglutaminase using a monoclonal-antibody immunoadsorbent.

• IKURA Koji

  Department of Food Science and Technology, Faculty of Agriculture, Kyoto University

• SAKURAI Hiroshi

  Department of Food Science and Technology, Faculty of Agriculture, Kyoto University

• OKUMURA Katsuzumi

  Department of Food Science and Technology, Faculty of Agriculture, Kyoto University

• SASAKI Ryuzo

  Department of Food Science and Technology, Faculty of Agriculture, Kyoto University

• CHIBA Hideo

  Department of Food Science and Technology, Faculty of Agriculture, Kyoto University

抄録

Guinea pig liver transglutaminase was purified in a yield of more than 80% by a one-step purification procedure using an immunoadsorbent column of monoclonal antibodies. Active enzyme could be recovered by alkaline buffer desorption and quick neutralization. The purified enzyme was more than 98% homogeneous on polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate, and had the same enzymological and immunological properties as those of the enzyme purified by conventional procedures.

収録刊行物

• Agricultural and Biological Chemistry

  Agricultural and Biological Chemistry 49 (12), 3527-3531, 1985

  公益社団法人 日本農芸化学会

キーワード

• General Agricultural and Biological Sciences
• General Biochemistry, Genetics and Molecular Biology

詳細情報

• CRID: 1390001206463289600
• NII論文ID: 130000026536
• DOI: 10.1271/bbb1961.49.3527
• COI: 1:CAS:528:DyaL28XkslKjsw%3D%3D
• ISSN: 18811280
• ISSN: 00021369
• ISSN: http://id.crossref.org/issn/00021369
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可