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- SHIOZAKI Shozo
- Department of Agricultural Chemistry, Kyoto University the Nippon Zeon Research Center
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- SHIMIZU Sakayu
- Department of Agricultural Chemistry, Kyoto University
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- YAMADA Hideaki
- Department of Agricultural Chemistry, Kyoto University
書誌事項
- タイトル別名
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- Unusual intracellular accumulation of S-adenosyl-L-methionine by microorganisms.
- Unusual accumulation of S-adenosyl-l-methionine by microorganisms
抄録
In survey studies on microbial accumulation of S-adenosyl-L-methionine (AdoMet), various yeasts were found to accumulate AdoMet intracellularly to a high concentration when they were grown in medium containing L-methionine. A group of sake yeasts (Saccharomyces sake) exhibited especially high accumulation. Of these yeasts, S. sake Kyokai No. 6 (K-6), which exhibited the highest accumulation, produced 12.6 μmol (5.03 mg) of AdoMet/ml broth. Almost all AdoMet produced was accumulated in cells, extracellular accumulation of AdoMet being very low. The maximum content of AdoMet of cells was 5.31 μmol (205 mg)/g dry cells. This was the highest value that had been reported. Methionine adenosyltransferase (EC 2.5.1.6) activity was significantly higher in this yeast compared to those in other yeasts tested. Ultraviolet photomicrographic studies on S. sake K-6 suggested that AdoMet was gradually accumulated in vacuoles with the passage of cultivation time.
収録刊行物
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- Agricultural and Biological Chemistry
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Agricultural and Biological Chemistry 48 (9), 2293-2300, 1984
公益社団法人 日本農芸化学会
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キーワード
詳細情報 詳細情報について
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- CRID
- 1390282681441274368
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- NII論文ID
- 130000026719
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- COI
- 1:CAS:528:DyaL2cXlvVOltro%3D
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- ISSN
- 18811280
- 00021369
- http://id.crossref.org/issn/00021369
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可