Quantitative study of anometric forms of glucose produced by α-glucosidase and glucoamylases

• CHIBA Seiya

  Department of Agricultural Chemistry, Faculty of Agriculture, Hokkaido University

• KIMURA Atsuo

  Department of Agricultural Chemistry, Faculty of Agriculture, Hokkaido University

• MATSUI HIROKAZU

  Department of Agricultural Chemistry, Faculty of Agriculture, Hokkaido University

書誌事項

タイトル別名

• Studies on anomeric forms of products of carbohydrases. Part I. Quantitative study of anomeric forms of glucose produced by .ALPHA.-glucosidases and glucoamylases.

抄録

A gas-liquid chromatographic method was applied to the determination of anomeric forms of sugar produced by carbohydrases. Anomeric forms of glucose released from maltotriose, phenyl amaltoside and phenyl a-glucoside were determined quantitatively. Thirteen a-glucosidases tested, including a-glucosidase from honey bee and acid α-glucosidase from pig's liver, were found to produce a-glucose exclusively, and two kinds of glucoamylases, only β-glucose. This method proved very useful for the determination of the anomeric forms of sugar produced. It was confirmed that mammalian acid a-glucosidase does not belong to the category of exo-1, 4-α-glucosidase (EC 3.2.1.3).

収録刊行物

• Agricultural and Biological Chemistry

  Agricultural and Biological Chemistry 47 (8), 1741-1746, 1983

  公益社団法人 日本農芸化学会

キーワード

• General Agricultural and Biological Sciences
• General Biochemistry, Genetics and Molecular Biology

詳細情報

• CRID: 1390282681439819776
• NII論文ID: 130000027318
• DOI: 10.1271/bbb1961.47.1741
• COI: 1:CAS:528:DyaL3sXltFygtb0%3D
• ISSN: 18811280
• ISSN: 00021369
• ISSN: http://id.crossref.org/issn/00021369
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可