Construction of a Yeast Strain with Regulatable Phospholipid Synthesis for Analysis of the Uptake and Metabolism of Phosphatidylethanolamine with Short Acyl Chains
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- Haraguchi Tomokazu DENG Lan
- Department of Biotechnology, The University of Tokyo
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- Nomura Keiichi KAKIHARA Toru
- Department of Biotechnology, The University of Tokyo
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- Yagi Fumio FUKUDA Ryouichi
- Department of Biotechnology, The University of Tokyo
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- OHTA Akinori
- Department of Biotechnology, The University of Tokyo
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Author(s)
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- Haraguchi Tomokazu DENG Lan
- Department of Biotechnology, The University of Tokyo
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- Nomura Keiichi KAKIHARA Toru
- Department of Biotechnology, The University of Tokyo
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- Yagi Fumio FUKUDA Ryouichi
- Department of Biotechnology, The University of Tokyo
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- OHTA Akinori
- Department of Biotechnology, The University of Tokyo
Abstract
Cycad leaf lectin (CRLL), a mannose-recognizing jacalin-related lectin (mJRL), was first cloned as a gymnosperm lectin and expressed. The cDNA sequence of CRLL (DDBJ, accession no. AB198328), coding 291 amino acid residues, has a tandem repeat of about 150 amino acids divided into N- and C-terminal domains as Japanese chestnut mJRL. Sequence alignment showed deletion and insertion of the sequence, and its putative carbohydrate-binding sites showed some differences from other JRLs. PCR analysis showed that this lectin was expressed in the cycad leaf but not in the root or seed. Recombinant CRLL (rCRLL) was expressed in <I>Escherichia coli</I> and purified by affinity chromatography after refolding procedures. Properties of active rCRLL appeared to be almost the same as those of native CRLL.
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 71(9), 2313-2315, 2007-09-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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